Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells.

BioMed Research International
Wanjuan XueJiming Han

Abstract

The study is aimed at investigating the role of Nei endonuclease VIII-like1 (NEIL1) in the pathogenesis of colorectal cancer (CRC). The human CRC (HCT116 and SW480) cells were subjected to the siRNA silencing and recombinant plasmid overexpression of NEIL1. Transfection of siNEIL1 significantly inhibited the cell growth. It also increased the Bax expression levels, while it decreased the Bcl-2 expression levels in human CRC cells, leading the Bax/Bcl-2 balance toward apoptosis. Moreover, the apoptosis was promoted through the caspase-9 signaling pathway. One the other hand, high expression of NEIL1 promoted the cell viability and reduced the apoptosis, inducing the balance of Bax/Bcl-2 in the human colon cancer cells to be antiapoptotic. In addition, the caspase-9 signaling pathway inhibited apoptosis, contrary to the results obtained by downregulating NEIL1 expression. Furthermore, NEIL1 was negatively regulated by miR-7-5p, indicating that miR-7-5p inhibited the NEIL1 expression after transcription. Overexpression of miR-7-5p reversed the effects of NEIL1 on these CRC cells. In conclusion, NEIL1 promotes the proliferation of CRC cells, which is regulated negatively by miR-7-5p. These findings suggest that NEIL1 is a potential...Continue Reading

References

Aug 21, 2004·Carcinogenesis·Kazuya ShinmuraHaruhiko Sugimura
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Jan 23, 2016·World Journal of Gastrointestinal Oncology·Wan-Qun ChenHai-Xia Deng
Apr 5, 2016·Oxidative Medicine and Cellular Longevity·Kazuya ShinmuraHaruhiko Sugimura
Dec 9, 2016·MicroRNA·Marissa WilliamsGlen Reid
Jun 6, 2019·American Journal of Physiology. Cell Physiology·Shawna DowningEmmanuel S Tzanakakis

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Citations

Jun 10, 2021·Molecular Genetics & Genomic Medicine·Yuming ZhengMeiling Zhu

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Methods Mentioned

BETA
glycosylation
Assay
PCR
flow cytometry
transfection

Software Mentioned

Syngene

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