Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae)

Antonie van Leeuwenhoek
Gui ZhangJianguo Xu

Abstract

Two Gram-stain negative, catalase positive, coccus shaped bacteria, designated 10023T and 10010, were isolated from the rectal contents of a plateau pika (Ochotona curzoniae) in Qinghai-Tibet Plateau, China. Based on 16S rRNA gene sequence analysis, phylogenetic trees showed that these two isolates (10023T, 10010) group with members of the genus Neisseria. Additionally, these two isolates exhibited high 16S rRNA gene sequence similarity with Neisseria zalophi CSL 7565T (96.98%), Neisseria wadsworthii WC 05-9715T (96.92%) and Neisseria canis ATCC 14687T (96.79%). Further phylogenetic analysis based on the rplF gene showed that these two novel strains can be easily discriminated from phylogenetically closely related species. Optimal growth was found to occur on BHI agar with 5% defibrinated sheep blood at 37 °C and growth was also observed on nutrient agar, Columbia blood agar and chocolate agar plates; however, growth was not observed on MacConkey agar after 7 days. The major cellular fatty acids of these strains were identified as C16:0 and C16:1ω7c/C16:1ω6c. The complete genome size of the type strain 10023T is 2,496,444 bp, with DNA G+C content of 54.0 mol %. The average nucleotide identity values were 73.5-79.3% between isol...Continue Reading

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Citations

Jun 5, 2019·International Journal of Systematic and Evolutionary Microbiology·Gui ZhangJianguo Xu
Feb 27, 2020·International Journal of Systematic and Evolutionary Microbiology·Zhi TianJianguo Xu
Apr 21, 2020·International Journal of Systematic and Evolutionary Microbiology·Gui ZhangJianguo Xu
Aug 26, 2020·International Journal of Systematic and Evolutionary Microbiology·Yajun GeJianguo Xu
Feb 2, 2022·International Journal of Systematic and Evolutionary Microbiology·Gui ZhangJianguo Xu

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Datasets Mentioned

BETA
KX088452
CP022278

Software Mentioned

MEGA7
MIDI
GeneMarkS
MEGA
EMBOSS
Blast
WebLogo
Single Molecule , Real - Time ( SMRT ) Analysis
Clustal W

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