NEK2 Promotes Hepatoma Metastasis and Serves as Biomarker for High Recurrence Risk after Hepatic Resection.

Annals of Hepatology
Yu-Ying ChangWenya Huang

Abstract

Developing reliable biomarkers for hepatocellular carcinoma (HCC) patients who are at a high risk of recurrence after curative hepatic resection is very important for determining subsequent therapeutic strategies. We investigated the role of the cell cycle factor NIMA-related kinase 2 (NEK2) in HCC progression in hepatoma cells and post-surgery patients. The effects of NEK2 on proliferation, invasion and migration of hepatoma HuH7 and SK-Hep1 cells were evaluated. In a post-surgery HCC cohort (N = 97), the Nek2 induction levels in the tumors were examined with real-time RT-PCR analysis, and the results were analyzed for their correlations with recurrence. NEK2 promoted G1 to S phase cell cycle progression by causing increases in cyclin D1 and AKT phosphorylation and decreases in the cyclin-dependent kinase inhibitor p27, indicating that NEK2 plays an important role during interphase in addition to its previously identified role in M phase. NEK2 also enhanced the proliferation, migration and invasion of hepatoma cells and regulated the expression of E-cadherin and MMP9. The Nek2 mRNA levels in the tumors were highly correlated with recurrence rates in the post-surgery HCC patients. Combined evaluation of the tumor AJCC stage and...Continue Reading

Citations

Oct 3, 2019·Experimental Biology and Medicine·Shirley Jusino, Harold I Saavedra
Jun 27, 2019·World Journal of Gastrointestinal Oncology·Ke-Wei Wang, Ming Dong
Jun 18, 2020·Frontiers in Oncology·Meiliang LiuXiaoyun Zeng
Aug 29, 2019·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Shanshan LiuBurong Li
Sep 4, 2020·Cancers·Anna Vidal, Torben Redmer
Feb 20, 2021·Experimental Biology and Medicine·Stephanie Colón-MarreroHarold I Saavedra

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