Nek2A specifies the centrosomal localization of Erk2

Biochemical and Biophysical Research Communications
Yang LouXuebiao Yao

Abstract

Nek2A is a cell-cycle-regulated protein kinase that localizes to the centrosome and kinetochore. Our recent studies provide a link between Nek2A and spindle checkpoint signaling [J. Biol. Chem. 279 (2004) 20049]. Extracellular signal-regulated kinase 2 (Erk2) is an important kinase, which belongs to mitogen activating protein (MAP) kinase family. Here we demonstrated that Nek2A binds specifically to Erk2. Erk2 interacts with Nek2A via a conserved Erk2 docking site located to the C-terminus of Nek2A. Our studies indicate this docking site is essential and sufficient for a direct Nek2A-Erk2 interaction. In addition, our immunocytochemical studies show that Nek2A and Erk2 are co-localized to centrosome. Significantly, elimination of Nek2A by RNA interference delocalized Erk2 from its centrosomal location, while inhibition of Erk2 kinase activity did not affect the localization of Nek2A in centrosome. We propose that Erk2 links extracellular signaling to centrosome dynamics by Nek2A.

References

Oct 21, 1998·Journal of Cellular Biochemistry·S O KimS L Pelech
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J H WrightE G Krebs
Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M G Wilkinson, J B Millar
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·E A Nigg
May 14, 2003·Trends in Cell Biology·Matthew J O'ConnellTony Hunter
Jun 25, 2003·The Journal of Cell Biology·Melinda M Horne, Thomas M Guadagno
Jul 15, 2003·Molecular Biology of the Cell·Alison J Faragher, Andrew M Fry
Sep 25, 2003·The Journal of Biological Chemistry·Fan-Li ChouMark H Ginsberg
Dec 31, 2003·Experimental Cell Research·Jae Cheal YooKunsoo Rhee

❮ Previous
Next ❯

Citations

Nov 2, 2011·Cell Division·Larissa MonizVuk Stambolic
Jul 5, 2011·International Journal for Parasitology·Tineke LauwaetFrances D Gillin
Dec 28, 2016·Cytoskeleton·Juliette Morlon-GuyotWassim Daher
Sep 30, 2020·Cells·Rustem E Uzbekov, Tomer Avidor-Reiss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.