Nek5 interacts with mitochondrial proteins and interferes negatively in mitochondrial mediated cell death and respiration

Cellular Signalling
Talita D Melo HanchukJörg Kobarg

Abstract

Mitochondria are involved in energy supply, signaling, cell death and cellular differentiation and have been implicated in several human diseases. Neks (NIMA-related kinases) represent a family of mammal protein kinases that play essential roles in cell-cycle progression, but other functions have recently been related. A yeast two-hybrid (Y2H) screen was performed to identify and characterize Nek5 interaction partners and the mitochondrial proteins Cox11, MTX-2 and BCLAF1 were retrieved. Apoptosis assay showed protective effects of stable hNek5 expression from Hek293-T's cell death after thapsigargin treatment (2 μM). Nek5 silenced cells as well as cells expressing a "kinase dead" version of Nek5, displayed an increase in ROS formation after 4 h of thapsigargin treatment. Mitochondrial respiratory chain activity was found decreased upon stable hNek5expression. Cells silenced for hNek5 on the other hand presented 1.7 fold increased basal rates of respiration, especially at the electrons transfer steps from TMPD to cytochrome c and at the complex II. In conclusion, our data suggest for the first time mitochondrial localization and functions for Nek5 and its participation in cell death and cell respiration regulation. Stable expre...Continue Reading

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Citations

Jun 13, 2012·Free Radical Biology & Medicine·Micha M M WilhelmusJack van Horssen
Jan 25, 2019·Molecular Carcinogenesis·Jing PeiBenzhong Wang
Jul 16, 2019·Molecular Reproduction and Development·Yuan-Yuan LiZhiming Han
May 16, 2019·Journal of Cellular Biochemistry·Talita Diniz Melo-HanchukJörg Kobarg
May 6, 2020·Proteome Science·Andressa Peres de OliveiraJörg Kobarg
Jan 8, 2020·BMC Cancer·Talita Diniz Melo-HanchukJörg Kobarg
Mar 7, 2021·Cells·Isadora Carolina Betim PavanJörg Kobarg

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