NELF potentiates gene transcription in the Drosophila embryo.

PloS One
Xiaoling WangJ Peter Gergen

Abstract

A hallmark of genes that are subject to developmental regulation of transcriptional elongation is association of the negative elongation factor NELF with the paused RNA polymerase complex. Here we use a combination of biochemical and genetic experiments to investigate the in vivo function of NELF in the Drosophila embryo. NELF associates with different gene promoter regions in correlation with the association of RNA polymerase II (Pol II) and the initial activation of gene expression during the early stages of embryogenesis. Genetic experiments reveal that maternally provided NELF is required for the activation, rather than the repression of reporter genes that emulate the expression of key developmental control genes. Furthermore, the relative requirement for NELF is dictated by attributes of the flanking cis-regulatory information. We propose that NELF-associated paused Pol II complexes provide a platform for high fidelity integration of the combinatorial spatial and temporal information that is central to the regulation of gene expression during animal development.

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Citations

Feb 18, 2011·Current Opinion in Genetics & Development·Jian Li, David S Gilmour
Apr 12, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Barbara H Jennings
Jul 7, 2012·Biological Reviews of the Cambridge Philosophical Society·Shuang Liu, Yongguang Tao
Dec 4, 2012·Biochimica Et Biophysica Acta·Yuki YamaguchiHiroshi Handa
Feb 28, 2020·Nucleic Acids Research·Paula Climent-CantóFernando Azorín
Apr 29, 2015·Nature Communications·Roman KesslerFernando Azorín
Dec 5, 2020·Scientific Reports·Guillaume BordetAlexei V Tulin

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Methods Mentioned

BETA
ChIP
reverse transcribed PCR
Immuno-Precipitation
PCR
immunoprecipitation

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