Abstract
NELL-1 (NEL-like Protein 1) is an osteoinductive protein with increasing usage as a bone graft substitute in preclinical animal models. NELL-1 was first identified to have bone-forming properties by its overexpression in fusing cranial sutures. Since this time, addition of recombinant NELL-1 has been used to successfully induce bone formation in the calvarial, axial and appendicular skeleton. With increasing interest in the use of NELL-1 as a bone-graft substitute, we sought to examine the expression of NELL-1 in a wide spectrum of benign and malignant bone-forming skeletal tumors. Immunohistochemical expression was examined in human pathologic specimens. Quantitative RT-PCR evaluated NELL-1 expression among OS cell lines in vitro. Results showed NELL-1 expression in all bone tumors. Likewise, all OS cell lines demonstrated increased NELL-1 expression in comparison to non-lesional human bone marrow stromal cells. Among, benign bone tumors (osteoid osteoma and osteoblastoma), strong and diffuse staining was observed, which spatially correlated with markers of osteogenic differentiation. In contrast, a relative reduction in NELL-1 staining was observed in osteosarcoma, accompanied by increased variation between tumors. Among oste...Continue Reading
References
Feb 1, 1988·Cancer·H YoshikawaY Sakamoto
Oct 1, 1985·Cancer·H YoshikawaK Ono
Jan 20, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K TingM T Longaker
May 11, 2001·The New England Journal of Medicine·R M NeerB H Mitlak
Jun 8, 2002·Toxicologic Pathology·John L VahleJames B Nold
Oct 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Jean-Jacques BodyAnthony B Hodsman
Mar 16, 2006·Human Molecular Genetics·Jayashree DesaiCymbeline T Culiat
Aug 29, 2006·The American Journal of Pathology·Tara AghalooChia Soo
Sep 6, 2006·Gastroenterology·Yuriko MoriStephen J Meltzer
Oct 7, 2006·Experimental Cell Research·Tsukasa SotoboriKazuyuki Itoh
Apr 25, 2007·Oncogene·Z JinS J Meltzer
May 23, 2007·Bone·Robert L Jilka
Oct 8, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xiaoji LuoTong-Chuan He
Sep 19, 2009·Calcified Tissue International·K-H William LauSubburaman Mohan
Dec 24, 2009·Tissue Engineering. Part a·Min LeeBenjamin M Wu
Jun 10, 2010·Tissue Engineering. Part a·Weiming LiChia Soo
Jul 22, 2010·Journal of Dental Research·X ZhangC Soo
Oct 13, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xinli ZhangChia Soo
Dec 7, 2010·Tissue Engineering. Part a·Ronald K SiuChia Soo
Feb 3, 2011·Plastic and Reconstructive Surgery·Weiming LiChia Soo
Apr 2, 2011·The Journal of Bone and Joint Surgery. American Volume·Hannu T AroAlexandre Valentin
Jul 5, 2011·Biochemical and Biophysical Research Communications·Aaron W JamesChia Soo
Aug 30, 2011·Tissue Engineering. Part a·Marcelo O FreireHomayoun H Zadeh
Jul 17, 2012·International Orthopaedics·Feng ChenXinli Zhang
Jul 19, 2012·Journal of Cellular Biochemistry·Jia ShenChia Soo
Aug 11, 2012·Endocrinology and Metabolism Clinics of North America·Natalie E Cusano, John P Bilezikian
May 21, 2013·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Wei YuanJian Dong
Sep 12, 2014·International Orthopaedics·Alan NguyenAaron W James
Citations
Apr 6, 2016·Journal of Orthopaedics·Jia ShenAaron W James
Feb 2, 2018·Journal of Cellular Biochemistry·Huaxiang ZhaoFeng Chen
Oct 16, 2019·Journal of Dental Research·C LiC Soo
Jun 15, 2017·JCI Insight·Aaron W JamesChia Soo
Mar 23, 2019·Journal of Orthopaedics·Carolyn A MeyersAaron W James