Nematocidal activity of extracellular enzymes produced by the nematophagous fungus Duddingtonia flagrans on cyathostomin infective larvae

Veterinary Parasitology
Fabio R BragaJackson V Araújo

Abstract

Duddingtonia flagrans produces chitinases, however, optimization of the production of these enzymes still needs to be explored, and its nematocidal activity should still be the subject of studies. The objective of the present study was to optimize chitinase production, and evaluate the nematocidal activity of extracellular enzymes produced by the nematophagous fungus D. flagrans on cyathostomin infective larvae. An isolate from D. flagrans (AC001) was used in this study. For the production of enzymes (protease and chitinase), two different culture media were inoculated with AC001 conidia. Both enzymes were purified. The statistical Plackett-Burman factorial design was used to investigate some variables and their effect on the production of chitinases by D. flagrans. After that, the design central composite (CCD) was used in order to determine the optimum levels and investigate the interactions of these variables previously observed. Only two variables (moisture and incubation time), in the evaluated levels, had a significant effect (p<0.05) on chitinase production. The conditions of maximum chitinase activity were calculated, with the following values: incubation time 2 days, and moisture 511%. The protease and chitinase derive...Continue Reading

References

Feb 24, 2007·Applied Microbiology and Biotechnology·Jinkui YangKe-Qin Zhang
Mar 10, 2010·Tropical Animal Health and Production·Fabio R BragaLaércio A Benjamin
Nov 23, 2013·Applied Microbiology and Biotechnology·Fabio Ribeiro Braga, Jackson Victor de Araújo

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Citations

Feb 2, 2017·Veterinary Research Communications·Thallyta Maria VieiraEduardo Robson Duarte
Nov 1, 2018·Brazilian Journal of Biology = Revista Brasleira De Biologia·E H GomesJ H Queiroz
Mar 30, 2017·Tropical Animal Health and Production·Gabriela Almeida BastosEduardo Robson Duarte
May 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcella PasqualettiMassimiliano Fenice

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