Neobiosynthesis of glycosphingolipids by plasma membrane-associated glycosyltransferases.

The Journal of Biological Chemistry
Pilar M CrespoJose Luis Daniotti

Abstract

Gangliosides, complex glycosphingolipids containing sialic acids, are synthesized in the endoplasmic reticulum and in the Golgi complex. These neobiosynthesized gangliosides move via vesicular transport to the plasma membrane, becoming components of the external leaflet. Gangliosides can undergo endocytosis followed by recycling to the cell surface or sorting to the Golgi complex or lysosomes for remodeling and catabolism. Recently, glycosphingolipid catabolic enzymes (glycohydrolases) have been found to be associated with the plasma membrane, where they display activity on the membrane components. In this work, we demonstrated that ecto-ganglioside glycosyltransferases may catalyze ganglioside synthesis outside the Golgi compartment, particularly at the cell surface. Specifically, we report the first direct evidence of expression and activity of CMP-NeuAc:GM3 sialyltransferase (Sial-T2) at the cell surface of epithelial and melanoma cells, with membrane-integrated ecto-Sial-T2 being able to sialylate endogenously synthesized GM3 ganglioside as well as exogenously incorporated substrate. Interestingly, we also showed that ecto-Sial-T2 was able to synthesize GD3 ganglioside at the cell surface using the endogenously synthesized ...Continue Reading

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Citations

Apr 25, 2012·Journal of Inherited Metabolic Disease·Massimo AureliSandro Sonnino
Jul 20, 2011·The Journal of Biological Chemistry·Aldo A VilcaesJose L Daniotti
Dec 24, 2013·Molecular Neurobiology·Sandro SonninoAlessandro Prinetti
Dec 25, 2010·Neurochemical Research·Massimo AureliSandro Sonnino
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Mar 5, 2011·Journal of Neurochemistry·Hugo J F MaccioniMariana L Ferrari
Jun 24, 2011·IUBMB Life·Jose Luis Daniotti, Ramiro Iglesias-Bartolomé
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Alessandro MaginiCarla Emiliani
Jun 28, 2016·Biochimica Et Biophysica Acta·Feng JiaChristopher W Cairo
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Aug 8, 2015·The Biochemical Journal·Macarena Rodriguez-WalkerJosé L Daniotti
Jan 1, 2012·ISRN Biochemistry·Thomas Kolter
Mar 21, 2019·International Journal of Molecular Sciences·Alessandro MaginiBrunella Tancini
Feb 9, 2020·International Journal of Molecular Sciences·Aldo A VilcaesJose L Daniotti

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