Neocortical efferent neurons with very slowly conducting axons: strategies for reliable antidromic identification

Journal of Neuroscience Methods
Harvey A Swadlow

Abstract

Although simple in concept, reliable antidromic identification of efferent populations poses numerous technical challenges and is subject to a host of sampling biases, most of which select against the detection of the neurons with slowly conducting axons. This problem is particularly acute in studies of the neocortex. Many neocortical efferent systems have large sub-populations with very slowly conducting, nonmyelinated axons and these elements have been relatively neglected in antidromic studies of neocortical neurons. The present review attempts to redress this problem by analyzing the steps that must necessarily precede antidromic identification and the sampling biases associated with each of these steps. These steps include (1) initial recognition that the microelectrode is near a neuron; (2) activation of the efferent axon via the stimulating electrode; (3) conduction of the antidromic impulse from stimulation site to soma; (4) detection of the antidromic spike in the extracellular record and (5) discriminating antidromic from synaptic activation. Experimental strategies are suggested for minimizing the sampling biases associated with each of these steps; most of which can be reduced or eliminated by appropriate experiment...Continue Reading

References

Oct 1, 1976·Experimental Neurology·S G Waxman, H A Swadlow
Oct 28, 1976·Experimental Brain Research·Y ShinodaH Asanuma
Dec 22, 1976·Experimental Brain Research·H AsanumaP Zarzecki
Jan 22, 1990·The Journal of Comparative Neurology·A S Lamantia, P Rakic
Mar 19, 1973·Experimental Brain Research·H Asanuma, I Rosén
Nov 1, 1970·Experimental Neurology·A L Towe, G W Harding
Jan 1, 1970·Brain, Behavior and Evolution·S H ChungJ Y Lettvin
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·H A SwadlowS G Waxman
Aug 11, 1980·Brain Research·G Aston-JonesF E Bloom
Jan 1, 1980·Experimental Brain Research·H F Moyano, J C Molina
Aug 15, 1980·Experientia·G L CollingridgeN K MacLeod
Apr 1, 1963·Journal of Neurology, Neurosurgery, and Psychiatry·R W GILLIATT, R G WILLISON

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Citations

Mar 22, 2006·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Shy ShohamRonen Segev
Sep 6, 2002·Nature·Richard H R HahnloserMichale S Fee
Feb 15, 2011·Nature Methods·Yoan LeChasseurYves De Koninck
Jan 8, 2013·Nature Neuroscience·Lindsey L GlickfeldR Clay Reid
Feb 13, 2010·Neuro-oncology·Marie-Thérése StockhausenHans Skovgaard Poulsen
May 23, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nicole R Kinzeler, Susan P Travers
Apr 24, 2009·Journal of Neurophysiology·Scott K ArfinRahul Sarpeshkar
Sep 9, 2011·Journal of Neurophysiology·Nancy F DayTeresa A Nick
Jun 20, 2008·Journal of Neurophysiology·Marie E Hemelt, Asaf Keller
Jan 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ernest E Kwegyir-Afful, Daniel J Simons
Jul 12, 2014·Annual Review of Biomedical Engineering·Melissa R WardenKarl Deisseroth
Sep 10, 2011·Journal of Neuroscience Methods·Yisi ZhangAlexay Kozhevnikov
Aug 18, 2004·Annals of the New York Academy of Sciences·Michale S FeeRichard H R Hahnloser
Dec 27, 2011·Neuron·Mark L AndermannR Clay Reid
Sep 3, 2010·PLoS Computational Biology·Botond Szatmáry, Eugene M Izhikevich
May 25, 2005·Journal of Neuroscience Methods·Bogdan NeaguDavid O Carpenter
Nov 5, 2004·Journal of Neurophysiology·I N BeloozerovaT G Deliagina
Oct 21, 2005·Journal of Neurophysiology·Mikhail G SirotaIrina N Beloozerova
Feb 24, 2006·Journal of Neurophysiology·Richard H R HahnloserMichale S Fee
Aug 24, 2007·Journal of Neurophysiology·Demetris S Soteropoulos, Stuart N Baker
Oct 29, 2013·Journal of Neural Engineering·Monzurul AlamEduardo Fernandez
Oct 27, 2018·Cerebral Cortex·Alexander KraskovRoger Lemon
Jun 24, 2017·Scientific Reports·Patrícia MeirelesMiguel Prudêncio
Feb 7, 2020·Cerebral Cortex·A KraskovS N Baker
May 31, 2002·Journal of Neurophysiology·Todd W TroyerKenneth D Miller
Oct 25, 2014·Cold Spring Harbor Protocols·Tatsuo S OkuboMichale S Fee

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