Neonatal estrogen stimulates proliferation of periductal fibroblasts and alters the extracellular matrix composition in the rat prostate

Endocrinology
W Y ChangGail S Prins

Abstract

The purpose of this study was to examine whether changes in extracellular matrix (ECM) molecules are associated with the growth inhibition and differentiation defects of the prostate gland following neonatal exposure to estradiol. Using immunocytochemistry (ICC), laminin and collagen IV were localized to the basement membrane (BM) as well to the basal lamina of the periductal smooth muscle of the control developing prostates. In contrast, fibronectin and collagen III were localized throughout the stromal ECM. Exposure to neonatal estrogen altered the staining profile for specific ECM molecules. In the estrogenized rats, a thick layer of cells negative for laminin and collagen IV was observed adjacent to the BM. Electron microscopy and ICC for alpha-actin, fibronectin, and vimentin identified this multicellular layer of periductal cells as differentiated fibroblasts. Peripheral to these fibroblasts, actin-positive smooth muscle formed a second layer of periductal stromal cells. PCNA labeling showed that estrogen exposure increased the fibroblast proliferation. Because many periductal fibroblasts were positive for estrogen receptor alpha (ER alpha) in estrogenized rats, a direct effect of estradiol on their proliferation is sugge...Continue Reading

References

Aug 1, 1991·Biology of Reproduction·N HayashiG R Cunha
Aug 1, 1990·Endocrine Reviews·R H GetzenbergD S Coffey
Apr 1, 1989·European Journal of Biochemistry·R Timpl
Apr 1, 1988·Biology of Reproduction·M J WilsonR D Estensen
Aug 1, 1987·Endocrine Reviews·G R CunhaY Sugimura
May 1, 1972·The American Journal of Anatomy·C J Flickinger
Jan 1, 1981·The Prostate·A Mariotti, M Mawhinney
Jan 1, 1994·The Prostate·R SanttiJ A McLachlan
Mar 1, 1995·Microscopy Research and Technique·M J Wilson
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill
Apr 30, 1996·Annals of the New York Academy of Sciences·S W HaywardR Dahiya
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·F S vom SaalW V Welshons
Apr 1, 1997·British Journal of Urology·S W HaywardG R Cunha

❮ Previous
Next ❯

Citations

Mar 27, 2012·Reproductive Biology and Endocrinology : RB&E·Samara U OlivaSandra M Miraglia
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yu-Ching PengAlexandra L Joyner
Apr 3, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Paras B SinghFrancis L Martin
Feb 3, 2015·Developmental Biology·Yu-Ching Peng, Alexandra L Joyner
May 9, 2008·Differentiation; Research in Biological Diversity·Gail S Prins, Oliver Putz
Oct 11, 2007·Differentiation; Research in Biological Diversity·William A RickeGerald R Cunha
Feb 11, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Leo FefermanJoanne K Tobacman
Aug 31, 2000·Endocrinology·R A JarredG P Risbridger
Sep 29, 2009·Journal of Dental Research·M Q WangA Raustia
Nov 26, 2002·Endocrinology·Joseph John BiancoGail Petuna Risbridger
Jul 1, 1999·The Prostate·W Y Chang, G S Prins
Oct 3, 2001·Physiological Reviews·S NilssonJ A Gustafsson
Jan 23, 2021·Differentiation; Research in Biological Diversity·Gail S Prins
Dec 21, 2002·Differentiation; Research in Biological Diversity·Gerald R CunhaY Z Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.