Neonatal hyperoxia disrupts the intestinal barrier and impairs intestinal function in rats

Experimental and Molecular Pathology
Hsiu-Chu Chou, Chung-Ming Chen

Abstract

Animal studies have demonstrated that neonatal hyperoxia injures the distal small intestine and disrupts the intestinal barrier. This study evaluated the effects of brief hyperoxia exposure on intestinal function in newborn rats. Newborn Sprague-Dawley rat pups were exposed to room air or normobaric hyperoxia (85% O2) for 1week. The rats were euthanized on Postnatal Day 7, and their terminal ilea and sera were collected for histological analyses and intestinal permeability measurements, respectively. Bacterial translocation to the liver and spleen under aerobic and anaerobic conditions was determined. The expression and localization of epithelial injury markers [intestinal fatty acid binding protein (I-FABP)], intestinal barrier proteins [occludin and zonula occludens (ZO)-1], and inflammation biomarkers [Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB)] were analyzed through immunofluorescence staining. The body weight at birth was comparable between the two groups. On Postnatal Day 7, the rats in the hyperoxic group exhibited significantly lower body weights, higher intestinal injury scores, lower numbers of goblet cells, higher I-FABP expression, lower occludin and ZO-1 expression, higher TLR4 and NF-κB expression, ...Continue Reading

Citations

Dec 2, 2017·American Journal of Reproductive Immunology : AJRI·Robert M Dietz, Clyde J Wright
Dec 19, 2017·Critical Care Medicine·Hadrien WiniszewskiGilles Capellier
May 16, 2019·Pediatric Research·Chung-Ming Chen, Hsiu-Chu Chou
Jan 12, 2021·BioMed Research International·D Y LiuS Y Sun
Jan 27, 2021·Journal of the Chinese Medical Association : JCMA·Yu-Chun LoChung-Ming Chen
Jun 3, 2021·Pediatric Pulmonology·Kun YangWenbin Dong
Jun 17, 2019·Experimental and Molecular Pathology·Hsiu-Chu Chou, Chung-Ming Chen

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