Neonatal immunization with a Sindbis virus-DNA measles vaccine induces adult-like neutralizing antibodies and cell-mediated immunity in the presence of maternal antibodies

The Journal of Immunology : Official Journal of the American Association of Immunologists
Alejandra V E CapozzoMarcela F Pasetti

Abstract

Infants younger than age 9 mo do not respond reliably to the live attenuated measles vaccine due the immaturity of their immune system and the presence of maternal Abs that interfere with successful immunization. We evaluated the immune responses elicited by Sindbis virus replicon-based DNA vaccines encoding measles virus (MV) hemagglutinin (H, pMSIN-H) or both hemagglutinin and fusion (F, pMSINH-FdU) glycoproteins in neonatal mice born to naive and measles-immune mothers. Despite the presence of high levels of maternal Abs, neonatal immunization with pMSIN-H induced long-lasting, high-avidity MV plaque reduction neutralization (PRN) Abs, mainly IgG2a, that also inhibited syncytium formation in CD150(+) B95-8 cells. IgG secreting plasma cells were detected in spleen and bone marrow. Newborns vaccinated with pMSINH-FdU elicited PRN titers that surpassed the protective level (200 mIU/ml) but were short-lived, had low syncytium inhibition capacity, and lacked avidity maturation. This vaccine failed to induce significant PRN titers in the presence of placentally transferred Abs. Both pMSIN-H and pMSINH-FdU elicited strong Th1 type cell-mediated immunity, measured by T cell proliferation and IFN-gamma production, that was unaffected...Continue Reading

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Citations

Dec 8, 2010·Expert Review of Anti-infective Therapy·Arnaud Gagneur, Didier Pinquier
Oct 11, 2008·Expert Review of Vaccines·Rory D de VriesRik L de Swart
Jun 15, 2012·The Pediatric Infectious Disease Journal·Maria Machaira, Vassiliki Papaevangelou
Apr 30, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weijuan ZhangSidong Xiong
Mar 18, 2016·Journal of Virology·Emily Julik, Jorge Reyes-Del Valle
Sep 22, 2017·Viruses·Emily Julik, Jorge Reyes-Del Valle

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