PMID: 6980238Mar 1, 1982Paper

Neonatal screening for 21-hydroxylase deficiency: a microfilter paper method for 17-alpha-hydroxyprogesterone assay

Journal of Endocrinological Investigation
S PiazziE Cacciari

Abstract

A micromethod for measuring 17 alpha-hydroxyprogesterone in blood collected on filter paper has been developed. Our method is rapid, easy and has the specificity, accuracy and precision of the radioimmunoassay in whole blood. The method has been applied for screening patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. Fifty samples collected on filter paper were assayed by our method, using 125I as tracer, and results were compared with those obtained for the same samples using a tritium tracer. The agreement between the two methods was particularly good in the area ranging from 15 to 100 pg/disc. In one neonate the diagnosis of CAH was made utilizing the microfilter paper method. Our method is a promising screening test for CAH. An indication of the advantages or disadvantages of this type of screening will become available when an adequate number of infants has been examined.

References

Jul 1, 1975·Clinical Endocrinology·E Youssefnejadian, R David
Jan 1, 1979·Journal of Endocrinological Investigation·G E Bacon, R P Kelch
Mar 1, 1971·The Journal of Clinical Endocrinology and Metabolism·A P SimpoulosF C Bartter
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Jun 6, 1963·The New England Journal of Medicine·A M BONGIOVANNI, A W ROOT

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Citations

Jan 1, 1986·Journal of Inherited Metabolic Disease·J L DhondtC Courte
Jan 1, 1986·Journal of Inherited Metabolic Disease·E CacciariM Capelli
Dec 2, 2008·Analytical and Bioanalytical Chemistry·Ralph Fingerhut, Bernhard Olgemöller
Apr 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·B A el-Gamal, J Landon
Jan 1, 1985·Annals of the New York Academy of Sciences·E CacciariM Capelli
Jul 1, 1983·Archives of Disease in Childhood·J F MurphyI A Hughes
Jan 1, 1984·Critical Reviews in Toxicology·G F RushJ B Hook
Oct 1, 1983·Archives of Disease in Childhood·E CacciariM Capelli

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