Neonatal testis growth recreated in vitro by two-dimensional organ spreading

Biotechnology and Bioengineering
Kazuaki KojimaTakehiko Ogawa

Abstract

Organ culture experiments can be hampered by central degeneration or necrosis due to the inadequate permeation of oxygen and nutrients, which deteriorates the function and growth of cultured tissues. In the current study, we aimed to overcome this limitation of organ culture through spreading the tissue two dimensionally on an agarose gel stand and molding into a disc shape by placing a ceiling of polydimethylsiloxane (PDMS) chip, which is highly oxygen permeable. By this, every part of the tissue can receive a sufficient supply of oxygen through PDMS as well as nutrients through the agarose gel below. This method not only prevented central necrosis of tissues, but also supported the tissue growth over time. In addition, such growth, as volume enlargement, could be easily measured. Under these conditions, we examined the effect of several factors on the growth of neonatal mouse testis, and found that follicle stimulating hormone (FSH) and insulin significantly promoted the growth. These results are in good agreement with previous in vivo reports. Notably, the growth achieved over 7 days in our in vitro system is almost comparable to, about 80% of, that observed in vivo. Thus, we successfully monitored the promotion of tissue gr...Continue Reading

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Citations

Jan 23, 2019·Biomaterials Science·Tohid Rezaei TopraggalehHossein Baharvand
Nov 7, 2019·International Journal of Molecular Sciences·Maxime VermeulenChristine Wyns
Mar 29, 2019·Biofabrication·Yoni BaertEllen Goossens
Oct 15, 2019·Reproductive Medicine and Biology·Mitsuru KomeyaTakehiko Ogawa
Nov 17, 2020·The Journal of Clinical Investigation·J Wolter Oosterhuis, Leendert Hj Looijenga
Feb 12, 2021·Scientific Reports·Takafumi MatsumuraTakehiko Ogawa
Oct 1, 2021·Science·Mitinori Saitou, Katsuhiko Hayashi

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Methods Mentioned

BETA
chip
chips
flow cytometry

Software Mentioned

AutoCAD
Leica Application Suite

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