Neonatal Type II Alveolar Epithelial Cell Transplant Facilitates Lung Reparation in Piglets With Acute Lung Injury and Extracorporeal Life Support

Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies
Xiuhua WangBo Sun

Abstract

Type II alveolar epithelial cells have potential for lung growth and reparation. Extracorporeal membrane oxygenation is used as life support for lung impairment resulting from acute respiratory distress syndrome. We hypothesized that intratracheal transplantation of isogeneic primary type II alveolar epithelial cells in combination with extracorporeal membrane oxygenation may facilitate lung reparation for acute lung injury (ALI). A randomized, controlled experiment. An animal laboratory in a university pediatric center. Twenty-eight 4- to 6-week young piglets, weighing 7-8 kg. Type II alveolar epithelial cells from neonatal male piglet lungs were isolated, purified, cultured, and labeled with chemical stain PKH26. After 3-6 hours of induction of ALI by IV endotoxin and mechanical ventilation (MV), young female piglets were allocated to five groups (n = 5): ALI-MV, ALI treated with MV; ALI-EC, ALI treated with both MV and venovenous extracorporeal membrane oxygenation; ALI-EC-T, ALI-EC protocol plus intratracheal type II alveolar epithelial cell transplant; CON-MV, healthy animals treated with MV; and CON-EC-T, healthy animals treated with venovenous extracorporeal membrane oxygenation. After 24 hours, animals were weaned from ...Continue Reading

References

Jul 1, 1991·American Journal of Respiratory Cell and Molecular Biology·M C WilliamsR L Hamilton
Jul 1, 1996·The Annals of Thoracic Surgery·R J NovickJ Lee
Aug 12, 1998·American Journal of Respiratory and Critical Care Medicine·G F ZhuB Robertson
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·Z H ZhouL W Zhu
Mar 25, 2000·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·S A MurphyR J Richards
Nov 29, 2002·Stem Cells·Nadir Askenasy, Daniel L Farkas
Sep 21, 2005·American Journal of Respiratory Cell and Molecular Biology·Michael A Matthay, Guy A Zimmerman
Feb 25, 2006·The Journal of Physiology·Jiwang ChenLin Liu
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dachun WangRick A Wetsel
Mar 14, 2009·Journal of Intensive Care Medicine·Joseph E LevittMichael A Matthay
May 7, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joanna RejmanMassimo Conese
Oct 14, 2009·JAMA : the Journal of the American Medical Association·UNKNOWN Australia and New Zealand Extracorporeal Membrane Oxygenation (ANZ ECMO) Influenza InvestigatorsMarc Ziegenfuss
Nov 11, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·R Britt McILwainAkhil Maheshwari
Jan 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dachun WangRick A Wetsel
Oct 13, 2010·Annual Review of Pathology·Michael A Matthay, Rachel L Zemans
Jul 2, 2011·The European Respiratory Journal·P SpitalieriF Sangiuolo
Jan 27, 2012·British Medical Bulletin·Orquidea GarciaDavid Warburton
Mar 8, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Allison N LauDaniel J Weiss
Jun 16, 2012·Journal of Cellular and Molecular Medicine·Zhao-hui QinLong Yu
Oct 2, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lavinia IonescuBernard Thébaud
Dec 12, 2012·Biochimica Et Biophysica Acta·Andrew E Vaughan, Harold A Chapman
Apr 13, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Ying-Gang ZhuJae-Woo Lee
Jul 23, 2013·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Yuanyuan QiLibo Sun
Nov 1, 2013·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Andrew A RosenbergLena M Napolitano
Dec 11, 2013·Current Opinion in Critical Care·Pierpaolo TerragniV Marco Ranieri

❮ Previous
Next ❯

Citations

Nov 1, 2016·Cell and Tissue Research·Sean E GillRuud A W Veldhuizen
Nov 16, 2018·Frontiers in Genetics·Lawrence S Prince
Mar 27, 2019·Intensive Care Medicine Experimental·Jonathan E MillarUNKNOWN National Health Medical Research Council Australia Centre of Research Excellence for Advanced Cardio-respiratory Therapies I

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.