Neonate human remains: a window of opportunity to the molecular study of ancient syphilis.

PloS One
Rafael MontielAssumpció Malgosa

Abstract

Ancient DNA (aDNA) analysis can be a useful tool in bacterial disease diagnosis in human remains. However, while the recovery of Mycobacterium spp. has been widely successful, several authors report unsuccessful results regarding ancient treponemal DNA, casting doubts on the usefulness of this technique for the diagnosis of ancient syphilis. Here, we present results from an analysis of four newborn specimens recovered from the crypt of "La Ermita de la Soledad" (XVI-XVII centuries), located in the province of Huelva in the southwest of Spain. We extracted and analyzed aDNA in three independent laboratories, following specific procedures generally practiced in the aDNA field, including cloning of the amplified DNA fragments and sequencing of several clones. This is the most ancient case, reported to date, from which detection of DNA from T. pallidum subspecies pallidum has been successful in more than one individual, and we put forward a hypothesis to explain this result, taking into account the course of the disease in neonate individuals.

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Citations

Jul 25, 2014·Proceedings. Biological Sciences·Marc SimónAssumpció Malgosa
Dec 24, 2014·Journal of Human Evolution·Kelly M Harkins, Anne C Stone
May 18, 2017·Annals of Human Biology·Charlotte J HouldcroftSimon J Underdown
Jun 22, 2018·PLoS Neglected Tropical Diseases·Verena J SchuenemannJohannes Krause
Apr 18, 2019·Journal of Medical Microbiology·Avril MeffrayPhilippe Biagini
May 23, 2015·Wiener medizinische Wochenschrift·Johanna Sophia GaulKarl Grossschmidt
Jan 21, 2020·American Journal of Physical Anthropology·Brenda J BakerStevie Winingear
Jul 1, 2020·Microbial Genomics·Nicolas Arning, Daniel J Wilson
Oct 12, 2016·Microbiology Spectrum·Jessica I Rivera-PerezGary A Toranzos
Aug 17, 2020·Current Biology : CB·Kerttu MajanderVerena J Schuenemann

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Methods Mentioned

BETA
PCR
or electron
X-ray

Software Mentioned

Blast

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