PMID: 118456Nov 1, 1979

Neoplastic transformation of epithelial cells in whole mammary gland in vitro

Proceedings of the National Academy of Sciences of the United States of America
N T TelangA B Kundu

Abstract

Biological characteristics of nodule-like alveolar lesions (NLAL) induced by 7,12-dimethylbenz[a]anthracene (DMBA) in organ culture of whole mammary gland (BALB/c female mice) were assessed after transplantation into gland-free mammary fat pads of syngeneic virgin mice. (i) Tissue-fragment explants from NLAL areas of the gland produced abnormal lobuloalveolar (LA) outgrowths in 3 of 10 fat pads. (ii) Transplantation of dissociated cells of NLAL-derived LA outgrowths into 36 fat pads showed 100% LA outgrowths and 3 (8%) of these 36 outgrowths produced mammary carcinomas. (iii) The explants of dissociated cells from whole mammary glands treated with DMBA in culture produced full or partial LA structures in 2 of 56 outgrowths. (iv) The explants of dissociated cells prepared from outgrowths derived from outgrowths derived from explants as in iii produced 9 LA outgrowths in 16 instances; mammary tumor incidence in these outgrowths was 3 of 16 (18%). (v) The explants of tissue fragments from LA outgrowths as in iv produced LA outgrowths in 20 of 20 fat pads; mammary carcinomas appeared in 16 of 20 (80%) of these outgrowths. No NLAL was detectable in control glands treated with dimethyl sulfoxide (solvent for DMBA); explants of the co...Continue Reading

References

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Citations

Jan 1, 1993·Journal of Cellular Biochemistry. Supplement·G J KelloffP Greenwald
Jan 1, 1996·Journal of Cellular Biochemistry. Supplement·V E SteeleG J Kelloff
Mar 1, 1989·Breast Cancer Research and Treatment·N T TelangM P Osborne
May 17, 2002·The Journal of Nutritional Biochemistry·Rajendra G Mehta, Rajeshwari R Mehta
Jul 7, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R G Mehta
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M S DickensS Sorof
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S MiyamotoS Nandi
Jul 19, 2001·Journal of the National Cancer Institute·R G MehtaJ M Pezzuto
Apr 30, 1996·Annals of the New York Academy of Sciences·N T Telang
Apr 1, 1987·European Journal of Cancer & Clinical Oncology·R MenonM R Banerjee
Sep 30, 1995·Annals of the New York Academy of Sciences·R ArakiN T Telang
Jun 15, 1981·International Journal of Cancer. Journal International Du Cancer·Q J TonelliS Sorof
Jun 15, 1987·International Journal of Cancer. Journal International Du Cancer·S ChakrabortyM R Banerjee
Jan 1, 1992·Journal of Cellular Biochemistry. Supplement·N T TelangM P Osborne
Sep 16, 2010·Cancer Prevention Research·Rajeshwari R MehtaTapas K Das Gupta

Related Concepts

Neoplastic Cell Transformation
Mammary Neoplasms, Experimental
Mice, Inbred BALB C
Neoplasm Transplantation
Organ Culture Techniques
9,10-Dimethyl-1,2-benzanthracene

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