Neopterin and 7,8-dihydroneopterin induce apoptosis in the rat alveolar epithelial cell line L2

FEBS Letters
W SchobersbergerD Fuchs

Abstract

The neopterin derivatives, neopterin and 7,8-dihydroneopterin, modulate the cellular oxidant-antioxidant balance as well as the expression of the inducible nitric oxide synthase (iNOS) gene. Since apoptosis can be induced by reactive oxygen intermediates and nitric oxide (NO) we investigated whether these neopterin derivatives induce apoptotic cell death. As model we selected the rat alveolar epithelial cell line L2. 24 h incubation of neopterin (1-1000 microM) as well as 7,8-dihydroneopterin (1-1000 microM) resulted in a significant increase of percent apoptotic cells (measured by FACS analysis). Coincubation of both pteridines with the cytomix (interferon-gamma plus tumor necrosis factor-alpha) led to a significantly higher apoptosis than the cytomix alone. In contrast to the cytomix, no iNOS gene expression and no NO release could be detected after incubation with neopterin or 7,8-dihydroneopterin. We conclude that neopterin and 7,8-dihydroneopterin are per se inducers of apoptosis which is not mediated by nitric oxide. This may be of importance in inflammatory pulmonary diseases associated with an activation of the cellular immune system.

References

Jan 1, 1992·Free Radical Research Communications·R SchreckP A Baeuerle
Nov 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Larrick, S C Wright
Sep 1, 1974·In Vitro·W H Douglas, M E Kaighn
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Dec 14, 1995·Biochemical and Biophysical Research Communications·G HoffmannW Schobersberger
Dec 1, 1994·International Archives of Allergy and Immunology·R Sgonc, G Wick
Jan 1, 1994·Immunology Today·T M Buttke, P A Sandstrom
Mar 1, 1993·Immunology Today·J J Cohen
Jan 1, 1993·International Archives of Allergy and Immunology·D FuchsH Wachter
Oct 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·G Baier-BitterlichD Fuchs

❮ Previous
Next ❯

Citations

Mar 5, 2003·Medical Hypotheses·Josef RiederGeorg Hoffmann
Jun 14, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Barbara WirleitnerDietmar Fuchs
Sep 23, 2006·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·M GulumianJ Murray
Feb 1, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·B MelicharR S Freedman
Jan 6, 2006·Biological & Pharmaceutical Bulletin·Emiko KanbeShin Aizawa
Dec 31, 2009·Indian Journal of Occupational and Environmental Medicine·Shubhangi K PingleAruna A Jawade
Jun 14, 2003·Redox Report : Communications in Free Radical Research·Steven P Gieseg, Sara Cato
Jun 22, 2000·Journal of Molecular and Cellular Cardiology·J MargreiterW Schobersberger
Oct 25, 2006·Journal of Thrombosis and Haemostasis : JTH·G Hoffmann
Oct 19, 2004·Molecular Immunology·Barbara WirleitnerDietmar Fuchs
May 14, 2005·Biochimica Et Biophysica Acta·Agnes BaloghJosef Margreiter
Oct 20, 2015·Journal of Psychiatric Research·Karina GhisoniAlexandra Latini
Dec 6, 2018·The Journal of Biological Chemistry·Satoshi HaraHiroshi Ichinose
Sep 1, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hiroshi AsaumiMakoto Otsuki
May 24, 2014·The Journal of Biological Chemistry·Heidi ContrerasCelia W Goulding
Jun 29, 2018·Antioxidants·Steven P GiesegAngus Lindsay
Mar 10, 1999·The American Journal of Physiology·R K MallampalliG A Koretzky
May 28, 2003·International Archives of Occupational and Environmental Health·Zeynep Zubeyde AltindagGonul Sahin
Dec 14, 1999·Clinical and Experimental Immunology·G HoffmannW Schobersberger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis