Neospora caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice

Experimental Parasitology
Houshuang ZhangXuenan Xuan

Abstract

Liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (M3-DPPE), referred to as M3-DPPE liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. To evaluate whether these M3-DPPE liposomes have an adjuvant capacity against Neospora caninum infection, a novel immunization method utilizing soluble N. caninum apical membrane antigen 1 (NcAMA1) encapsulated in the M3-DPPE liposomes (M3-NcAMA1) was employed. The results revealed that a significant amount of interferon (IFN)-gamma production was detected in culture supernatants of NcAMA1 protein- or N. caninum lysate-stimulated spleen cells obtained from the mice one week after the third immunization with M3-NcAMA1. The parasite burden in the dams' brain tissue was decreased and the survival rate of offspring increased significantly in M3-NcAMA1-immunized mice. Thus, a parasite-specific Th1 immune response was successfully induced in the pregnant mice immunized with M3-NcAMA1, and an effective reduction of offspring mortality from N. caninum infection was triggered.

References

Dec 2, 1996·Veterinary Parasitology·J P Dubey, D S Lindsay
Nov 14, 1997·Immunology Today·R Raghupathy
Aug 24, 1999·Veterinary Parasitology·J P Dubey
Dec 23, 1999·International Journal for Parasitology·T V BaszlerB A Mathison
Oct 12, 2001·International Journal for Parasitology·E A InnesD Buxton
Sep 12, 2002·Biochimica Et Biophysica Acta·Lucy East, Clare M Isacke
Apr 2, 2003·The Korean Journal of Parasitology·J P Dubey, John Dubey
Apr 16, 2003·American Journal of Veterinary Research·R M O'HandleyJ P Dubey
May 30, 2006·The Journal of Parasitology·Noritaka KubokiChihiro Sugimoto
Oct 26, 2007·Clinical and Vaccine Immunology : CVI·Penglong HuangXuenan Xuan
May 20, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Naoya KojimaKunio Tsujimura

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Citations

Mar 8, 2014·Experimental Parasitology·Thierry Monney, Andrew Hemphill
Jul 10, 2010·Journal of Biomedicine & Biotechnology·Yuzuru IkeharaTakashi Yamaguchi
Feb 22, 2016·Infectious Diseases of Poverty·Yongzhi ZhouJinlin Zhou
Sep 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Damer P BlakeFiona M Tomley
Jan 1, 2011·Animals : an Open Access Journal From MDPI·Thierry MonneyAndrew Hemphill

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