Neovasculogenic therapy to augment perfusion and preserve viability in ischemic cardiomyopathy

The Annals of Thoracic Surgery
Pavan AtluriY Joseph Woo

Abstract

Ischemic cardiomyopathy is a global health concern with limited therapy. We recently described endogenous revascularization utilizing granulocyte-macrophage colony stimulating factor (GMCSF) to induce endothelial progenitor cell (EPC) production and intramyocardial stromal cell-derived factor-1alpha (SDF) as a specific EPC chemokine. The EPC-mediated neovascularization and enhancement of myocardial function was observed. In this study we examined the regional biologic mechanisms underlying this therapy. Lewis rats underwent left anterior descending coronary artery (LAD) ligation and developed ischemic cardiomyopathy over 6 weeks. Three weeks after ligation, the animals received either subcutaneous GMCSF and intramyocardial SDF injections or saline injections as control. Six weeks after LAD ligation circulating EPC density was studied by flow cytometry. Quadruple immunofluorescent vessel staining for mature, proliferating vasculature was performed. Confocal angiography was utilized to identify fluorescein lectin-lined vessels to assess perfusion. Ischemia reversal was studied by measuring myocardial adenosine triphosphate (ATP) levels. Myocardial viability was assayed by terminal deoxynucleotidyl transferase-mediated deoxyuridin...Continue Reading

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Citations

May 9, 2008·Journal of Cardiothoracic Surgery·Pavan AtluriY Joseph Woo
Oct 30, 2008·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·C QianA J M Roks
Aug 21, 2012·Trends in Cardiovascular Medicine·William HiesingerY Joseph Woo
Aug 20, 2010·Heart, Lung & Circulation·Pavan AtluriY Joseph Woo
Apr 7, 2010·The Journal of Thoracic and Cardiovascular Surgery·J Raymond FitzpatrickY Joseph Woo
Feb 5, 2008·The Journal of Thoracic and Cardiovascular Surgery·Pavan AtluriY Joseph Woo
Jan 1, 2009·The International Journal of Medical Robotics + Computer Assisted Surgery : MRCAS·Pavan AtluriY Joseph Woo
Dec 19, 2012·The Journal of Thoracic and Cardiovascular Surgery·John W MacArthurY Joseph Woo
Aug 5, 2009·Cell Transplantation·Huey-Shan HungShinn-Zong Lin
Jan 19, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Kyoko HoboHiromi Kurosawa

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