Nepetoidin B, a Natural Product, Inhibits LPS-stimulated Nitric Oxide Production via Modulation of iNOS Mediated by NF-κB/MKP-5 Pathways

Phytotherapy Research : PTR
Xiaxia WuXiuping Chen

Abstract

Previous reports showed that nepetoidin B (NTB), a natural product isolated from many herbs, has anti-fungal and anti-bacterial effects. In this study, the antiinflammatory effect of NTB was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The cytotoxic effect of NTB and LPS was determined by MTT assay. The nitric oxide (NO) production was detected by Griess assay. The TNF-α and IL-6 levels were determined by enzyme-linked immunosorbent assay kits. Protein expressions were tested by western blotting. The transcription activity of inducible nitric oxide synthase (iNOS) was detected by luciferase assay. Immunofluorescence assay was used to observe the visualization of NF-κB/p65 nuclear translocation. NTB and LPS showed no obvious cytotoxic effect on RAW 264.7 cells. NTB remarkably inhibited LPS-induced NO and TNF-α secretion in a concentration-dependent manner while showed no significant effect on IL-6 secretion. NTB inhibited LPS-induced iNOS protein expression and transcription activity without affecting cyclooxygenase-2. Furthermore, NTB suppressed LPS-stimulated NF-κB/p65 phosphorylation and nuclear translocation. In addition, NTB significantly inhibited LPS-induced phosphorylation of JNK1/2 and p38M...Continue Reading

References

Jun 1, 1990·Chemical & Pharmaceutical Bulletin·T NakanishiM Wakashiro
Jan 27, 1999·Biochemical and Biophysical Research Communications·E D Chan, D W Riches
Jul 3, 1999·The Journal of Biological Chemistry·T TanoueE Nishida
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Sep 18, 2002·The EMBO Journal·Aristides G EliopoulosPhilip N Tsichlis
Mar 17, 2007·International Journal of Cardiology·Tsung O Cheng
Jan 6, 2011·Molecular Nutrition & Food Research·Alexander GosslauNancy E Rawson
Jan 18, 2011·Journal of Natural Medicines·Toshihiro MurataFumihiko Yoshizaki
Dec 7, 2011·Critical Reviews in Immunology·Manuela RossolSunna Hauschildt
Jan 22, 2013·Bioorganic & Medicinal Chemistry Letters·Guo-Cai WangWen-Cai Ye
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Oct 12, 2013·Cancer Treatment and Research·Barbora OrlikovaMarc Diederich
Oct 15, 2013·The American Journal of Chinese Medicine·Christian StumpfChristoph D Garlichs
Feb 25, 2014·Phytochemistry·Sheng-Fa Tsai, Shoei-Sheng Lee
Jul 15, 2015·Gastroenterology·Bruce E Sands
Feb 20, 2016·Circulation Research·Mary G Sorci-Thomas, Michael J Thomas

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