Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats

FEBS Letters
Shireen KahaiBurton B Yang

Abstract

We found that nephronectin was significantly down-regulated by TGF-beta1. To determine the function of nephronectin in osteogenesis, we generated various constructs to produce stable MC3T3-E1 cell lines, expressing and secreting nephronectin protein, including full-length (Npnt), lacking EGF-like repeats (Np-MAM), and lacking RGD and MAM domains (Np-EGF). We demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. Lack of these repeats resulted in inhibiting the change in morphology. Over-expression of nephronectin results in earlier formation of bone nodules than the vector control. ERK activation is essential for nephronectin-induced osteoblast differentiation.

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Citations

Feb 18, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·David A Long, Rachel Lennon
Jan 30, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Yuya SatoKiyotoshi Sekiguchi
Apr 26, 2014·International Journal of Experimental Pathology·Anna M Piccinini, Kim S Midwood
Jun 22, 2011·Biochemical and Biophysical Research Communications·Masayuki TsukasakiRyutaro Kamijo
Jul 31, 2012·Biochemical and Biophysical Research Communications·Masayuki TsukasakiRyutaro Kamijo
Jul 5, 2016·Biological & Pharmaceutical Bulletin·Tamaki KurosawaRyutaro Kamijo
Sep 20, 2016·FEBS Open Bio·Katsuhiro HiranumaRyutaro Kamijo
Feb 9, 2012·Journal of Proteome Research·Claire E MolinariPeter E Hartmann
Jan 18, 2017·Biochemical and Biophysical Research Communications·Mikiko IkehataRyutaro Kamijo
May 18, 2017·Journal of Cellular Physiology·Youqiang SunJiake Xu
Oct 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chengbo GuZhiguo Liu
Jan 24, 2018·Stem Cells International·Jia Tang, Takashi Saito
Aug 27, 2017·Pathology Oncology Research : POR·Sayra DilmacGamze Tanriover
Oct 21, 2018·Calcified Tissue International·Tadashi KatoRyutaro Kamijo
May 11, 2018·FEBS Open Bio·Tadashi KatoRyutaro Kamijo
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