Nerve growth factor and associated nerve sprouting contribute to local mechanical hyperalgesia in a rat model of bone injury

European Journal of Pain : EJP
M YasuiY Sugiura

Abstract

To clarify the mechanism of tenderness after bone injury, we investigated changes in the withdrawal threshold to mechanical stimuli, nerve distribution and nerve growth factor (NGF)-expression in a rat model of bone injury without immobilization for bone injury healing. Rats were divided into three groups as follows: (1) rats incised in the skin and periosteum, followed by drilling a hole in the tibia [bone lesion group (BLG)]; (2) those incised in the skin and periosteum without bone drilling [periosteum lesion group (PLG)]; and (3) those incised in the skin [skin lesion group (SLG)]. Mechanical hyperalgesia continued for 28 days at a lesion in the BLG, 21 days in PLG and 5 days in SLG after treatments, respectively. Endochondral ossification was observed on days 5-28 in BLG and on days 5-21 in PLG. Nerve growth appeared in deep connective tissue (DCT) at day 28 in BLG. Nerve fibres increased in both cutaneous tissue and DCT at day 7 in PLG, but they were not found at day 28. Mechanical hyperalgesia accompanied with endochondral ossification and nerve fibres increasing at the lesion in both BLG and PLG. NGF was expressed in bone-regenerating cells during the bone injury healing. Anti-NGF and trk inhibitor K252a inhibited hyper...Continue Reading

References

Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Nov 3, 1998·Acta Orthopaedica Scandinavica·B L Grills, J A Schuijers
Aug 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J LiA Kreicbergs
Apr 24, 2004·Journal of Orthopaedic Trauma·Stephan ZeiterKeita Ito
Dec 6, 2005·Clinics in Sports Medicine·Michael C Koester, Kurt P Spindler
Mar 1, 2006·Somatosensory & Motor Research·Ken TakahashiKazue Mizumura
Nov 2, 2006·Prehospital and Disaster Medicine·Stamatios A PapadakisApostolos A Tentes
Jul 20, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nathan J KoewlerPatrick W Mantyh
May 2, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Simone D GueriosDale E Bjorling
May 12, 2009·Neuroscience Letters·Tomohiko NishigamiTakahiro Ushida
Nov 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juan M Jimenez-AndradePatrick W Mantyh

❮ Previous
Next ❯

Citations

Jul 6, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Olaf KilianKatrin Susanne Lips
Jul 25, 2014·European Journal of Pain : EJP·S FalkA M Heegaard
Dec 23, 2015·Neural Regeneration Research·Shao-Hua LiRan Huo
Aug 27, 2013·Nature Reviews. Drug Discovery·Frank M Longo, Stephen M Massa
Apr 29, 2019·Journal of Translational Medicine·Xubin QiuJinbo Liu
Oct 26, 2018·British Journal of Clinical Pharmacology·Patrick W Mantyh
Oct 14, 2017·The Journal of Physiological Sciences : JPS·Tatsuki NakagawaTomoko Koeda
Jun 28, 2018·Current Osteoporosis Reports·Stefanie A T MitchellPatrick W Mantyh
Aug 11, 2021·Experimental and Therapeutic Medicine·Huan YangYong Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.