Nerve growth factor and K-252a increase catecholamine release from PC12 cells

Journal of Neuroscience Research
B NikodijevicG Guroff

Abstract

PC12 cells are a nerve growth factor-responsive clone derived from a rat pheochromocytoma. The cells contain catecholamines and secrete them in response to depolarizing stimuli and cholinergic agonists. Treatment of the cells with nerve growth factor produces a number of very rapid changes, including the structural rearrangement of the cell membrane, the generation of a number of different second messengers, and the phosphorylation of several proteins. The present studies show that nerve growth factor treatment increases the release of dopamine and norepinephrine from the cells within a few minutes and does so independently of its effect on their metabolism. The experiments indicate that this effect on nerve growth factor is dependent on the presence of extracellular calcium and can be blocked by calcium channel antagonists. K-252a, an alkaloid-like material, usually found to inhibit the actions of nerve growth factor on PC12 cells, also increases the release of catecholamines under these conditions.

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Citations

Nov 19, 1997·Journal of Neuroscience Research·H Jiang, G Guroff
Feb 1, 1991·Journal of Neuroscience Research·B Nikodijevic, G Guroff
Mar 1, 1995·Journal of Neuroscience Research·B NikodijevicG Guroff
Aug 15, 1995·Journal of Neuroscience Research·L R de SouzaJ K Reed
Aug 27, 1993·Annals of the New York Academy of Sciences·G Guroff
Apr 1, 1995·The American Journal of Physiology·S PitchfordB S Glaeser

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