Nerve growth factor continuously elevates in a rat rotator cuff tear model

Journal of Shoulder and Elbow Surgery
Naoshige NaguraMasashi Takaso

Abstract

Nerve growth factor (NGF) plays a key role in osteoarthritic pain and low back pain. Rotator cuff tear (RCT) is often associated with severe shoulder pain. However, the role of NGF in RCT remains to be fully understood. Rats were divided into sham and RCT groups. The rotator cuff was harvested from the sham and RCT groups on various days for reverse transcription-polymerase chain reaction analysis of Tnfa, Ngf, Il1b, and Cox2 expression. Rotator cuffs from the sham and RCT groups were also harvested at 1 and 14 days for enzyme-linked immunosorbent assay and immunohistochemistry to assess NGF protein levels and localization. Rotator cuff-derived cells were stimulated with rat recombinant tumor necrosis factor (TNF)-α to investigate the involvement of TNF-α in the regulation of NGF expression. Tnfa and Ngf messenger RNA levels increased within 1 day in the RCT group. Notably, Tnfa and Ngf upregulation persisted for up to 56 days after the RCT surgery, while Il1b and Cox2 expression was significantly reduced. NGF levels in the RCT group were significantly higher than those in the sham operation group on days 1 and 14. Certain inflammatory cells and synovial-like cells lining the surface of the laminated tears were NGF-positive on ...Continue Reading

Citations

Jun 5, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Helen L MoserAlice H Huang
Jan 6, 2021·The Clinical Journal of Pain·Davide PrevitaliChristian Candrian

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