Nerve growth factor enhances cholinergic innervation and contractile response to electric field stimulation in a murine in vitro model of chronic asthma

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
O BacharL-O Cardell

Abstract

Asthma is a chronic inflammatory disease characterized by airway hyper-responsiveness. Alterations in the neurogenic control are believed to contribute to the pathogenesis. Yet, the long-term interaction between nerves and inflammatory mediators, such as the neurotrophin nerve growth factor (NGF), are not fully understood much due to the absence of appropriate experimental assays. To develop an ex vivo mouse organ culture assay and to investigate the effects of NGF on nerve-mediated airway contractions. Mouse tracheal segments were cultured in periods of up to 16 days. Their contractile responses to electric field stimulation (EFS) were investigated. In addition, the effect of 4 days of NGF treatment was analysed using EFS and immunohistochemistry. EFS (0.2-25.6 Hz) induced reproducible and frequency-dependent cholinergic contractions of both fresh and cultured tracheal segments. The main part of the EFS response was blocked by tetrodotoxin or atropine. After 4 days in culture, regional differences appeared, with stronger EFS responses in distal than in proximal segments. More nerve fibres were seen in distal segments than in proximal segments. Treatment with NGF during 4 days of culture increased the innervation of the proxima...Continue Reading

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Citations

Apr 26, 2005·Respiratory Physiology & Neurobiology·Ofir BacharLars-Olaf Cardell
Nov 11, 2009·American Journal of Respiratory Cell and Molecular Biology·Jenny PanAllen C Myers
Oct 5, 2007·Pharmacology & Therapeutics·V Freund-Michel, N Frossard
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