PMID: 8599177Oct 1, 1995Paper

Nerve growth factor from the venom of the Chinese cobra Naja naja atra: purification and description of non-neuronal activities

Toxicon : Official Journal of the International Society on Toxinology
T KostizaJ Meier

Abstract

Nerve growth factor (NGF) was separated from crude Naja naja atra venom by using weak cation-exchange chromatography, followed by reversed-phase liquid chromatography. The yield of the purification was 0.2-0.5% (w/w). The mol. wt was determined to be 13,600 and the protein still induced the typical fibre outgrowth of cultured PC-12 cells in a concentration range of 5-10 ng/ml. Beside this neuronal effect we demonstrated non-neuronal effects of cobra venom NGF, such as induction of plasma extravasation and histamine release from whole blood cells. With human leucocyte preparations, including enriched basophils, there was an increase in C5a-induced histamine release, whereas NGF alone was inactive. Cobra NGF was one-tenth as potent as human recombinant NGF, with a half-maximal stimulation occurring at 10 ng/ml. Cobra NGF and human recombinant NGF showed a modulatory effect on histamine release comparable to the haematopoietic growth factor IL-3. Thus, the non-neuronal effects of cobra NGF may account for immunomodulatory activities during inflammatory events.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N S OrensteinM Young
Apr 26, 1978·Biochimica Et Biophysica Acta·S Furukawa, K Hayashi
Nov 1, 1976·Journal of Biochemistry·S Furukawa, K Hayashi
Dec 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P J SmithC von Holt
Jan 1, 1991·European Journal of Immunology·H KimataH Mikawa
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·R M Kini, H J Evans
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B L ChanA R Saltiel
May 1, 1989·Journal of Neuroscience Research·B MorganJ R Perez-Polo
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·U OttenR Peck
Jan 1, 1987·Journal of Neuroscience Research·L W ThorpeJ R Perez-Polo
Jan 1, 1988·International Archives of Allergy and Applied Immunology·H MatsudaJ A Denburg
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H MatsudaJ A Denburg
Jan 1, 1987·Journal of Neuroscience Research·L W Thorpe, J R Perez-Polo
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J SiigurM Saarma
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R H Angeletti
Oct 30, 1984·European Journal of Pharmacology·U OttenJ Girard
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A P GeeM Young
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A K LiM Young
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P B EhrhardU Otten

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Citations

Jul 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·T Kostiza, J Meier
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liu-jiao BianXiao-yan Yang
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Steven D Aird
Dec 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Johara Boldrini-FrançaEliane Candiani Arantes
Mar 3, 2018·Toxins·Shu-Zhi Wang, Zheng-Hong Qin
Dec 17, 2011·Toxins·Tatjana MomicPhilip Lazarovici
Jul 1, 2004·The Biochemical Journal·Dawn C-I KohK Jeyaseelan

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