PMID: 6407016Jun 1, 1983Paper

Nerve growth factor in sympathetic ganglia and corresponding target organs of the rat: correlation with density of sympathetic innervation

Proceedings of the National Academy of Sciences of the United States of America
S Korsching, H Thoenen

Abstract

A two-site enzyme immunoassay is described which does not suffer from artifacts inherent in previous assays and has the necessary high sensitivity to determine the endogenous levels of nerve growth factor (NGF) in the sympathetic nervous system and its target organs. Monoclonal and affinity-purified polyclonal antibodies against mouse NGF (mNGF) were covalently linked to glass beads as the first site and coupled to the enzyme beta-galactosidase as the second site. Detection of the fluorescent beta-galactosidase reaction product permitted the determination of 0.01-0.02 fmol of mNGF per assay. The recovery of mNGF added to homogenates varied between 50% and 100%, depending on the tissue. Rat superior cervical and stellate ganglia were found to contain (mean +/- SEM) 25 +/- 4 and 19 +/- 3 ng of NGF per g wet weight, respectively, and the densely innervated submandibular gland, heart atrium, and iris contained 0.5 +/- 0.1, 1.0 +/- 0.1, and 1.9 +/- 0.3 ng of NGF per g wet weight, respectively. Heart ventricle and skeletal muscle, which are poorly innervated by the sympathetic nervous system, did not contain detectable levels of NGF (less than 0.3 ng/g wet weight). Serum contained less than 0.05 ng of NGF per ml. The correlation betw...Continue Reading

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Citations

Dec 1, 1991·The American Journal of Anatomy·B J TripathiN S Borisuth
Oct 1, 1992·Journal of Neuroscience Research·K MuraseK Hayashi
May 1, 1994·Journal of Neuroscience Research·Y MullerJ Clos
Sep 1, 1990·Cell and Tissue Research·K Seidl, A F Holstein
Mar 1, 1988·Cellular and Molecular Neurobiology·H ThoenenH Rohrer
Jun 1, 1991·Neurochemical Research·T S BurchamE M Shooter
Apr 1, 1986·Journal of Neurocytology·P J FinnR A Rush
Jan 1, 1987·Molecular Neurobiology·A D Lander
Apr 6, 2006·Archives of Dermatological Research·Ying-Chun DouOlle Johansson
Jan 30, 2010·In Vitro Cellular & Developmental Biology. Animal·Shimon LechtPeter I Lelkes
Jan 11, 1991·Brain Research·C ZettlerR A Rush
Feb 1, 1992·Developmental Biology·J A QianP Levitt
Feb 26, 1987·Journal of Immunological Methods·L Lärkfors, T Ebendal
Nov 1, 1984·Cell Differentiation·J P Thiery

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