Nerve growth factor-induced up-regulation of cytosolic phospholipase A2alpha level in rat PC12 cells

Neuroscience Letters
Nobuteru AkiyamaT Murayama

Abstract

Nerve growth factor (NGF) regulates various types of gene transcription in neurons. One of the cytosolic phospholipase A(2)s, cPLA(2)alpha, which preferentially cleaves phospholipids at the sn-2 position to arachidonic acid (AA), is involved in neuronal responses including survival. We investigated the effect of NGF on cPLA(2)alpha expression and its signaling pathways in PC12 cells, which differentiate into neuronal-like cells with neurites by NGF treatment. Treatment with NGF increased cPLA(2)alpha mRNA level after 4h and its protein level 24h after NGF addition. The NGF-induced increase in cPLA(2)alpha mRNA was inhibited by actinomycin D. NGF caused phosphorylation of mitogen-activated protein kinases (MAPKs); sustained phosphorylation of extracellular-regulated kinases (ERK1/2) and transient phosphorylation of p38 MAPK. NGF responses (cPLA(2)alpha mRNA and its protein) were inhibited by selective inhibitors for the ERK1/2 pathway, p38 MAPK and c-Jun NH(2)-terminal kinase. Epidermal growth factor, which transiently activates ERK1/2, did not modify cPLA(2)alpha expression. Although phorbol 12-myristate 13-acetate, an activator of protein kinase C (PKC), alone showed no effect, NGF-induced cPLA(2)alpha mRNA expression decrease...Continue Reading

References

Sep 12, 1998·The Journal of Biological Chemistry·T Morooka, E Nishida
Nov 18, 2000·Biochimica Et Biophysica Acta·T Hirabayashi, T Shimizu
Sep 18, 2001·The Journal of Biological Chemistry·S A BlaineR A Nemenoff
Jun 14, 2003·The Journal of Biological Chemistry·Salvador Naranjo-SuárezLuis del Peso
Feb 21, 2004·European Journal of Pharmacology·Ken ShimamuraToshihiko Murayama

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Citations

Oct 11, 2005·Annual Review of Cell and Developmental Biology·Joseph R Marszalek, Harvey F Lodish
Oct 24, 2006·Neuroscience Letters·Tomoko KoikeMakoto Makishima
Dec 22, 2011·The Journal of Biological Chemistry·Yongwoo JangUhtaek Oh
Mar 6, 2015·Journal of Basic and Clinical Physiology and Pharmacology·Keren EttingerPhilip Lazarovici

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