Nerve growth factor inhibits apoptosis induced by S-100 binding in neuronal PC12 cells

Neuroscience
S FulleG Fanò

Abstract

When grown for seven days in a medium containing nerve growth factor (100 ng/ml), 10% horse serum and 5% fetal bovine serum PC12 cells stopped dividing, extended neurites and assumed a neuronal phenotype. Withdrawal of nerve growth factor from these cells resulted in loss of neurites and apoptotic changes in many cells. The apoptotic changes were exacerbated if the cells were also exposed to 1-2 microM S-100, a calcium binding protein purified from bovine brain. After exposure to S-100, the PC12 cells underwent characteristic apoptotic changes. Within 2 in neurites retracted, the cell body shrunk and submembranous accumulation of condensed cytoplasmic material was observed. DNA ladders were present after 24-48 h and 60% of the cells became hypodiploid after 72 h. S-100 induced apoptosis by binding to specific sites (Kd = 189 nM) on PC12 cells and this caused a rise in [Ca2+]i due to a transmembrane capacitative flux followed by the depletion of internal stores. This increase was reversed if 5 microM nifedipine, a specific L-type Ca2+ channel inhibitor, was added to the medium after S-100 and completely abolished if the cells were pretreated with 5 microM thapsigargin, an inhibitor of endoplasmic reticulum Ca(2+)-ATPase. The pre...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jun 1, 1992·Current Opinion in Neurobiology·T P Snutch, P B Reiner
Jun 1, 1992·Current Opinion in Neurobiology·D C Lo
Aug 1, 1992·Trends in Neurosciences·J Altman
May 1, 1991·Trends in Neurosciences·H Thoenen
Jan 1, 1991·Annual Review of Neuroscience·R W Oppenheim
Dec 15, 1987·Brain Research·L J Van Eldik, D B Zimmer
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Kligman, D R Marshak
Jul 1, 1968·Physiological Reviews·R Levi-Montalcini, P U Angeletti
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·V Bocchini, P U Angeletti
May 1, 1994·Neuroscience·M A MariggióG Fanó
May 1, 1995·Progress in Neurobiology·G FanòP Calissano
Sep 29, 1994·Biochimica Et Biophysica Acta·L J Van Eldik, W S Griffin
Sep 30, 1994·Biochemical and Biophysical Research Communications·Y KobayashiT Shinozawa
May 1, 1994·Journal of Neuropathology and Experimental Neurology·L C StanleyW S Griffin
Jun 1, 1993·Current Opinion in Neurobiology·R PennerM Hoth

❮ Previous
Next ❯

Citations

Jun 25, 1999·Brain Research. Molecular Brain Research·S WangK G Haglid
Dec 18, 2003·Neurobiology of Disease·E J WolvetangP J Hertzog
Aug 9, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Oskar KarlssonMalin Andersson
Feb 9, 2011·Journal of Cellular Physiology·Karol OndriasOlga Krizanova
May 7, 2011·Brain Research·Vladimir O PustylnyakOleg I Epstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis