Nerve growth factor mRNA in brain: localization by in situ hybridization

Biochemical and Biophysical Research Communications
P D Rennert, G Heinrich

Abstract

Nerve Growth Factor is a 118 amino acid polypeptide that plays an important role in the differentiation and survival of neurons. The recent discovery that a mRNA that encodes beta Nerve Growth Factor is present in brain suggests that the Nerve Growth Factor gene may not only regulate gene expression of peripheral but also of central neurons. To identify the site(s) of Nerve Growth Factor mRNA production in the brain and to determine which cells express the Nerve Growth Factor gene, the technique of in situ hybridization was employed. A 32P-labeled RNA probe complementary to Nerve Growth Factor mRNA hybridized to cells in the stratum granulosum of the dentate gyrus and the stratum pyramidale of the hippocampus. These observations identify for the first time cellular sites of Nerve Growth Factor gene expression in the central nervous system, and suggest that Nerve Growth Factor mRNA is produced by neurons.

Citations

Jan 1, 1990·Molecular Neurobiology·J R Perez-PoloK Werrbach-Perez
Dec 1, 1988·Brain Research. Developmental Brain Research·C R BuckI B Black
Aug 1, 1989·Brain Research. Developmental Brain Research·F Gómez-PinillaM Nieto-Sampedro
May 31, 1993·Behavioural Brain Research·L E HarrellT S Barlow
Nov 1, 1989·Brain Research. Molecular Brain Research·T NishimoriG R Uhl
Oct 1, 1991·Brain Research. Molecular Brain Research·B LuI B Black
Jan 1, 1991·Brain Research. Molecular Brain Research·C GallP J Isackson
Jun 1, 1992·Brain Research. Molecular Brain Research·E Dicou
May 1, 1992·Brain Research. Molecular Brain Research·S ShimadaG Uhl
Apr 1, 1993·Brain Research. Molecular Brain Research·C CerrutiG R Uhl
Mar 1, 1994·Brain Research. Molecular Brain Research·C CerrutiM J Kuhar
Jul 1, 1994·Brain Research. Molecular Brain Research·I NeveuP Brachet
Jan 1, 1990·Progress in Neurobiology·P J Harrison, R C Pearson
Jan 1, 1992·Progress in Neurobiology·M Hayashi
Jun 1, 1993·Progress in Neurobiology·H HaraK Kogure
Apr 10, 1989·Neuroscience Letters·H LorezU Otten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.