PMID: 9191085Jun 1, 1997Paper

Nerve growth factor mRNA stability is controlled by a cis-acting instability determinant in the 3'-untranslated region

Brain Research. Molecular Brain Research
B TangB C Wise

Abstract

Nerve growth factor (NGF) mRNA is rapidly degraded in many non-neuronal cell types with a half-life of between 30 and 60 min. Similar to other short-lived mRNAs the 3'-untranslated region (3'-UTR) of the NGF mRNA contains a short AU nucleotide-rich sequence. To implicate this region as a cis-acting determinant of NGF mRNA instability, expression vectors containing NGF cDNA with and without the 3'-UTR, and vectors containing only the 3'-UTR were constructed and used in cell transfection experiments. Transfection of HEK293 or NIH3T3 cells with these expression vectors followed by measurement of NGF mRNA half-life indicated that NGF mRNA without the AU-rich 3'-UTR was approximately 3-fold more stable than NGF mRNA containing the 3'-UTR. Similar results were seen in a polysome-based cell-free RNA decay assay using NGF mRNA with and without the 3'-UTR prepared from transfected cells. Addition of a short RNA containing the AU-rich 3'-UTR to the cell-free RNA decay system prolonged the half-life of the full-length NGF mRNA, suggesting competition between these two RNA species for polysome-associated factors which degrade the NGF mRNA. Moreover, transfection of HEK293 or astroglial cells with vectors designed to express only the AU-ric...Continue Reading

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Citations

Dec 5, 1998·Brain Research. Molecular Brain Research·B Kavety, J I Morgan
Mar 9, 2002·Biochemical and Biophysical Research Communications·Shiro SudaNobufumi Kawai
Sep 14, 2007·Journal of Neuroscience Research·Federico Bolognani, Nora I Perrone-Bizzozero
Jun 13, 2006·Cell Calcium·Christine M MisquittaAshok K Grover
Feb 24, 2001·Journal of Endodontics·J S SullinsE F Wheeler
Jan 27, 2017·Journal of Cell Science·Thomas S Lisse, Sandra Rieger
Aug 8, 2006·Molecular Pharmaceutics·Pradeep TyagiLeaf Huang

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