Nerve growth factor of cultured medium extracted from human degenerative nucleus pulposus promotes sensory nerve growth and induces substance p in vitro

Spine
Kazuyo YamauchiSeiji Ohtori

Abstract

We investigated the mechanism of discogenic low back pain using an in vitro model. To evaluate the axonal growth and induction of a painful neuropeptide, substance P (SP), using rat dorsal root ganglion (DRG) neurons and degenerated human disc cells in vitro. Degeneration of the lumbar intervertebral disc is a cause of low back pain. The pathologic mechanism is thought to be sensory nerve ingrowth into the inner layers of the degenerated intervertebral disc; however, the precise patho-mechanism has not been clarified. The nucleus pulposus (NP) and annulus fibrosus (AF) of human intervertebral discs were harvested from patients with discogenic low back pain. Extracted medium from human degenerative intervertebral discs was cultured with neurons of rat DRGs. We evaluated the promotion of axonal growth and SP induction of DRG neurons in extracted medium from the NP and AF using immunocytochemistry. The average length of growing axons in the NP and AF was significantly longer than that in the control (P < 0.005). That in the NP was significantly longer than that in the AF. The average length of growing axons in the NP was significantly shortened after anti-nerve growth factor (NGF)beta treatment (P < 0.005); however, that in the AF...Continue Reading

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Citations

Aug 7, 2012·BMC Musculoskeletal Disorders·Andreas GerhardtWolfgang Eich
Sep 7, 2013·Philosophy, Ethics, and Humanities in Medicine : PEHM·Thor Eirik EriksenRani Lill Anjum
Oct 24, 2012·PloS One·Stephen M RichardsonJudith A Hoyland
Mar 1, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefania E NavoneEugenio A Parati
Mar 22, 2014·Journal of Cellular and Molecular Medicine·Emerson KrockLisbet Haglund
May 12, 2010·Journal of Anatomy·José García-CosamalónJosé A Vega
Mar 24, 2015·The Spine Journal : Official Journal of the North American Spine Society·Hyunchul KimAdam H Hsieh
Mar 25, 2014·The Spine Journal : Official Journal of the North American Spine Society·Seiji OhtoriKazuhisa Takahashi
May 8, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Helen E GruberEdward N Hanley
Jan 18, 2014·Global Spine Journal·D PurmessurJ C Iatridis
Jan 18, 2014·Global Spine Journal·Akihiko HiyamaJoji Mochida
Jul 3, 2013·Nature Reviews. Neurology·Bart Morlion
Feb 10, 2020·The Journal of Pain : Official Journal of the American Pain Society·John D MarkmanRoger Chou
Oct 11, 2011·Lancet·Federico BalaguéChristine Cedraschi

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