Nerve growth factor rescues PC12 cells from apoptosis by increasing amount of bcl-2

Biochemical and Biophysical Research Communications
S KatohT Suzuki

Abstract

Nerve growth factor (NGF) suppressed the decrease in number of viable PC12 cells after serum withdrawal from culture medium. Accordingly, the amount of bcl-2, a suppressive effector of apoptosis, increased in these cells. Bcl-2 antisense oligonucleotide suppressed not only the NGF-induced increase in bcl-2 but also NGF-induced neuronal differentiation. Results of fluorescent DNA staining indicated that NGF inhibited the chromatin condensation of PC12 cells resulting from serum withdrawal and further that the bcl-2 antisense oligonucleotide canceled this effect of NGF. The present results suggest that NGF rescues PC12 cells from apoptosis induced by serum withdrawal via up-regulation of bcl-2.

Citations

Jun 27, 2007·Neurochemical Research·Christopher Brynczka, Bruce Alex Merrick
Apr 15, 2010·Journal of Immunoassay & Immunochemistry·Makio Mogi, Ayami Kondo
Mar 8, 2007·Annals of the New York Academy of Sciences·Silvia CristofanonLina Ghibelli
Sep 15, 2004·Brain Research. Molecular Brain Research·William R SchelmanJames A Weyhenmeyer
Mar 14, 2000·Cell Death and Differentiation·K B Sandau, B Brüne
Apr 25, 2000·Oncogene·L LindenboimR Stein
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria D'AlessioLina Ghibelli
Oct 16, 1999·The Journal of Biological Chemistry·Z Feng, A G Porter
Feb 12, 1999·Brain Research. Developmental Brain Research·D M BradleyM B Heaton

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