PMID: 11922865Apr 2, 2002Paper

Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins

BMC Neuroscience
James M AngelastroLloyd A Greene

Abstract

NGF exerts a variety of actions including promotion of neuronal differentiation and survival. The PC12 rat pheochromocytoma cell line has proved valuable for studying how NGF works and has revealed that the NGF mechanism includes regulation of gene expression. Accordingly, we used SAGE (Serial Analysis of Gene Expression) to compare levels of specific transcripts in PC12 cells before and after long-term NGF exposure. Of the approximately 22,000 transcripts detected and quantified, 4% are NGF-regulated by 6-fold or more. Here, we used database information to identify transcripts in our SAGE libraries that encode ribosomal proteins and have compared the effect of NGF on their relative levels of expression. Among the transcripts detected in our SAGE analysis, 74 were identified as encoding ribosomal proteins. Ribosomal protein transcripts were among the most abundantly expressed and, for naive and NGF-treated PC12 cells, represented 5.2% and 3.5%, respectively, of total transcripts analyzed. Surprisingly, nearly half of ribosomal protein transcripts underwent statistically significant NGF-promoted alterations in relative abundance, with changes of up to 5-fold. Of the changes, approximately 2/3 represented decreases. A time course...Continue Reading

Citations

Nov 4, 2004·Human Mutation·Andrew Chatr-AryamontriFabrizio Loreni
Mar 19, 2008·Journal of Neurochemistry·Thomas F DijkmansErno Vreugdenhil
May 15, 2009·The FEBS Journal·Sara CaldarolaFabrizio Loreni
Apr 20, 2006·Animal Biotechnology·John D NeillElizabeth Liebler-Tenorio
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M MihmP J O'Shaughnessy
Jul 18, 2009·Journal of Neuroscience Research·Martine UittenbogaardAnne Chiaramello
Aug 11, 2007·Gene·Robert P Perry
Nov 18, 2008·Brain Research·T F DijkmansE Vreugdenhil

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