Nerve growth factor specifically stimulates translation of eukaryotic elongation factor 1A-1 (eEF1A-1) mRNA by recruitment to polyribosomes in PC12 cells.

The Journal of Biological Chemistry
Emmanuel Petroulakis, Eugenia Wang

Abstract

During postnatal brain development the level of peptide elongation factor-1A (eEF1A-1) expression declines and that of the highly homologous isoform, eEF1A-2, increases in neurons. eEF1A-1 is implicated in cytoskeletal interactions, tumorigenesis, differentiation, and the absence of eEF1A-2 is implicated in neurodegeneration in the mouse mutant, wasted. The translation of eEF1A-1 mRNA is up-regulated via mitogenic stimulation. However, it is not known if eEF1A-1 mRNA translation is regulated by neurotrophins or if its synthesis is differentially regulated than that of the neuronal isoform, eEF1A-2. Regulated translation of these factors by neurotrophins, particularly by the Trk class of neurotrophin receptors, would implicate them in differentiation, survival, and neuronal plasticity. In this study, we investigated the effect of nerve growth factor (NGF) stimulation on the synthesis of eEF1A-1 and eEF1A-2. We found that NGF stimulation causes a preferential synthesis of eEF1A-1 over eEF1A-2 in PC12 cells. We analyzed the co-sedimentation of eEF1A-1 mRNA with polyribosome fractions in sucrose gradients, and found that NGF stimulation enriched the presence of eEF1A-1 mRNA in polyribosomes, indicating that the translation of eEF1A...Continue Reading

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Citations

Aug 18, 2006·Psychopharmacology·Brice J WilliamsAnn-Charlotte Granholm-Bentley
Jul 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·Maricel Marin-KuanBenoît Schilter
Apr 18, 2008·Toxicology and Applied Pharmacology·Leire ArbillagaAdela López de Cerain
Jun 1, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Matthew L KrausharMladen-Roko Rasin
Oct 28, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·M Marin-KuanB Schilter
Jul 18, 2006·Neurochemical Research·Daniel B McClatchyAllan I Levey

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