PMID: 8612603Mar 1, 1996Paper

Nerve growth factor treatment of sensory neuron primary cultures causes elevated levels of the mRNA encoding the ATP synthase beta-subunit as detected by a novel PCR-based differential cloning method

European Journal of Biochemistry
G KendallD S Latchman

Abstract

The mRNA encoding the rat ATP synthase beta-subunit was rapidly induced by nerve growth factor, within 60 min, in cultured adult rat dorsal root ganglion neurons. ATP synthase beta-subunit cDNA clones were isolated from a lambda library. The library was constructed using rat dorsal root ganglion mRNA that was differentially screened with cDNA-derived probes from untreated and nerve-growth-factor-treated primary cultures of adult rat dorsal root ganglion sensory neurons. Radiolabelled probes were made from submicrogram quantities of RNA, by a novel PCR-based technique, which allows small amounts of primary tissue to be used for library screening. The use of this technique in isolating novel differentially expressed mRNAs is discussed.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Milbrandt
Mar 1, 1994·Brain Research. Molecular Brain Research·N N NinkinaJ N Wood
Jan 1, 1990·The European Journal of Neuroscience·R. M. LindsayD. Lindholm

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Citations

Nov 26, 2005·The European Journal of Neuroscience·Andrew MossMaria Fitzgerald

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