Nerve injury elevates functional Cav3.2 channels in superficial spinal dorsal horn

Molecular Pain
Xiao-Jin FengTao Liu

Abstract

Cav3 channels play an important role in modulating chronic pain. However, less is known about the functional changes of Cav3 channels in superficial spinal dorsal horn in neuropathic pain states. Here, we examined the effect of partial sciatic nerve ligation (PSNL) on either expression or electrophysiological properties of Cav3 channels in superficial spinal dorsal horn. Our in vivo studies showed that the blockers of Cav3 channels robustly alleviated PSNL-induced mechanical allodynia and thermal hyperalgesia, which lasted at least 14 days following PSNL. Meanwhile, PSNL triggered an increase in both mRNA and protein levels of Cav3.2 but not Cav3.1 or Cav3.3 in rats. However, in Cav3.2 knockout mice, PSNL predominantly attenuated mechanical allodynia but not thermal hyperalgesia. In addition, the results of whole-cell patch-clamp recordings showed that both the overall proportion of Cav3 current-expressing neurons and the Cav3 current density in individual neurons were elevated in spinal lamina II neurons from PSNL rats, which could not be recapitulated in Cav3.2 knockout mice. Altogether, our findings reveal that the elevated functional Cav3.2 channels in superficial spinal dorsal horn may contribute to the mechanical allodyni...Continue Reading

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Citations

Aug 11, 2020·Pain·Kimberly GomezRajesh Khanna
Jan 11, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Song CaiRajesh Khanna
Apr 20, 2021·British Journal of Pharmacology·Erika K HardingMichael E Hildebrand

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Methods Mentioned

BETA
transmission electron microscopy
PCR
electrophoresis
dissection
glycosylation

Software Mentioned

GraphPad Prism
Patchmaster
SPSS
Image J
GraphPad

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