PMID: 6162572Feb 1, 1981Paper

Nerve-specific enolase and creatine phosphokinase in axonal transport: soluble proteins and the axoplasmic matrix

Cell
S T Brady, R J Lasek

Abstract

The axonal transport of two soluble enzymes of intermediary metabolism was evaluated: the nerve-specific form of the glycolytic enzyme enolase (NSE) and the brain isozyme of creatine phosphokinase (CPK). Previously, little was known about the intracellular movements of the soluble proteins of the cell. Although the soluble enzymes of glycolysis and other pathways of intermediary metabolism have been thought to be freely diffusing in the cytosol, many are required in the axonal extremities of the neuron and must be transported to the sites of utilization. Comigration of purified enzymes with radioactive polypeptides associated with specific rate components of axonal transport in two-dimensional gel electrophoresis indicates that both NSE and CPK move in the axon solely as part of the group of proteins known as slow component b (SCb) at a rate of 2 mm/day. Peptide mapping following limited proteolysis confirmed identification of NSE and CPK in SCb. Materials associated with SCb have been shown to move coherently along the axon and to behave as a discrete cellular structure, the axoplasmic matrix. Association of two soluble enzymes, NSE and CPK, with the SCb complex of proteins requires a reevaluation of the assumption that these ...Continue Reading

References

Feb 23, 1976·Biochemical and Biophysical Research Communications·P J MarangosC York
May 5, 1975·Biochimica Et Biophysica Acta·P MarangosS C Bondy
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T Lorenz, M Willard
Jun 15, 1976·European Journal of Biochemistry·J Mowbray, V Moses
Jan 1, 1977·Current Topics in Cellular Regulation·J H Ottaway, J Mowbray
Nov 1, 1977·Cell·M Osborn, K Weber
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T WallimannH M Eppenberger
Jun 1, 1979·The Journal of Cell Biology·M WillardP Skene
Jan 12, 1978·Biochimica Et Biophysica Acta·H R Knull
Jun 16, 1978·Biochimica Et Biophysica Acta·E T Fossel, A K Solomon
Jan 13, 1975·Biochimica Et Biophysica Acta·F M Clarke, C J Masters
Jan 1, 1975·Methods in Enzymology·T Baranowski, E Wolna
May 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H ArnoldD Pette
Apr 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P Sigel, D Pette
May 1, 1971·Journal of Bioenergetics·G HübscherH J Hansen
Jul 17, 1974·Biochimica Et Biophysica Acta·F M Clarke, C J Masters
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M WillardP R Vagelos
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
May 1, 1968·Archives of Biochemistry and Biophysics·M D Joshi, V Jagannathan
Nov 1, 1968·European Journal of Biochemistry·H Arnold, D Pette
Mar 1, 1967·The American Journal of the Medical Sciences·J Eshchar, H J Zimmerman
Aug 1, 1980·The Journal of Cell Biology·M M Black, R J Lasek
Jan 1, 1981·The Journal of Cell Biology·J A Garner, R J Lasek
Jan 1, 1980·Journal of Neurochemistry·H R KnullW G Niehaus
Dec 1, 1958·Journal of Neurochemistry·N R ROBERTSE J CRAWFORD

❮ Previous
Next ❯

Citations

Jan 1, 1992·Molecular Neurobiology·J L Cyr, S T Brady
Jul 19, 2012·Bulletin of Experimental Biology and Medicine·K A PavlovV P Chekhonin
Mar 24, 1987·Brain Research·D J Gower, M Tytell
Nov 30, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversO R Hommes
Jan 1, 1990·The International Journal of Biochemistry·J W IrvineC L Saunderson
Jan 1, 1986·Journal of the Neurological Sciences·D J Gower, M Tytell
Jan 1, 1993·Progress in Biophysics and Molecular Biology·L A Fothergill-Gilmore, P A Michels
Jun 17, 1994·Brain Research. Developmental Brain Research·M Jousselin-HosajaF Rioux
Jan 1, 1985·Neurochemistry International·H R Knull
Jan 1, 1982·Neuroscience·S TsukitaH Ishikawa
Jun 1, 1987·Brain Research Bulletin·M WillardR Cheney
Feb 19, 2002·Neuroscience Research·Masaru YamamotoTakeshi Kato
Jan 13, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·O Ramírez, E Jiménez
Sep 12, 2001·The International Journal of Biochemistry & Cell Biology·F G MengH M Zhou
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S MonacoP Gambetti
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B D Clark, I R Brown
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F DillmanK K Pfister
Aug 1, 1988·The Journal of Cell Biology·K R Fath, R J Lasek
May 1, 1992·The Journal of Cell Biology·R J LasekM J Katz
Sep 1, 1982·The Journal of Cell Biology·B J Schnapp, T S Reese
Jul 1, 1984·The Journal of Cell Biology·R J LasekS T Brady
Nov 1, 1984·The Journal of Cell Biology·S T BradyR J Lasek
May 24, 2011·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·L S Nersesova
Jan 1, 1983·Annual Review of Biochemistry·L F Reichardt, R B Kelly
Oct 1, 1991·Reviews on Environmental Health·E A Smith, F W Oehme
Mar 4, 2014·Progress in Neurobiology·Jason R PlemelWolfram Tetzlaff
Jul 28, 2012·Journal of Neurogenetics·Aparna Sadananda, Krishanu Ray
Aug 1, 1994·Journal of Neuroscience Research·A KellerM Lucas
Feb 1, 1997·Kidney International·K K Graven, H W Farber
Apr 1, 2003·American Journal of Hematology·Roser López-AlemanyJordi Félez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.