Nervous system function for the testis RNA-binding protein boule in Drosophila

Journal of Neurogenetics
Mei-Ling Joiner, Chun-Fang Wu

Abstract

The RNA-binding protein, Boule is conserved across species and is required for male fertility. Boule and the DAZ homologues in mice and humans appear specific to the testis. Boule functions in spermatogenesis by controlling the translation of the meiotic cell division cycle 25 (Cdc25) phosphatase, Twine. Here we show, for the first time, a function for the DAZ protein, Boule, outside of meiosis. We found that an isoform of Boule is expressed in the nervous system and when its expression is increased we observe mutant phenotypes in neural communication between the receptor and laminar cells of the fly eye, altered larval locomotion and when further overexpressed, viability. As in the germ line, genetic studies indicate that Boule functions in the Cdc25 phosphatase pathway in the nervous system. In a sensitized genetic background of Boule overexpression, we added a loss-of-function mutation of twine and demonstrated a role for Twine Cdc25, in the adult nervous system. Our results indicate that isoforms of boule are expressed outside of the male germ line and that these isoforms have a role in neural function, unlike the boule testis-specific isoform.

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Citations

Jun 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric D HoopferLiqun Luo
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew BrookNicola K Gray
Jan 17, 2015·Developmental Biology·Kazuki SekinéMasatsugu Hatakeyama
Jan 1, 2011·Spermatogenesis·Michael J W Vangompel, Eugene Y Xu
Jul 12, 2011·Developmental Biology·Georg KualesPeter Ladurner
Nov 7, 2008·Molecular Genetics and Genomics : MGG·Qingbo ZhangZhuang Xie
Jul 20, 2006·The Biochemical Journal·Simon MortonSimon Rousseau

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