Nesfatin-1 ameliorates testicular injury and supports gonadal function in rats induced with testis torsion

Peptides
Sevil Arabaci TamerBerrak Ç Yeğen

Abstract

Testicular torsion causes ischemia-reperfusion injury and an increased risk of infertility. Nesfatin-1 is a novel peptide with antioxidant, anti-inflammatory and anti-apoptotic properties. In the present study, we aimed to investigate the putative beneficial effects of nesfatin-1 on oxidative injury and impaired testicular function induced by testis torsion. Under anesthesia, male Sprague-Dawley rats (180-230 g; n = 24) had sham-operation or they underwent testicular torsion by rotating the left testis 720° and fixing it for 2 h, followed by a 2-h detorsion. Rats in each group were treated intraperitoneally with either nesfatin-1 (0.3 μg/kg) or saline prior to the torsion or sham-torsion. At the end of the 4-h experimental period, tissue samples were removed for evaluation of spermatozoa, molecular and histochemical analyses. In saline-treated torsion/detorsion group, a high percentage of abnormal spermatozoa with head defects was observed, which was abolished in nesfatin-1-treated torsion/detorsion group. The levels of 8-OHdG, tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-3 were increased in the saline-treated torsion/detorsion group as compared to sham-operated group, while nesfatin-1 pre-treatment significan...Continue Reading

Citations

Nov 28, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Walaa Yehia AbdelzaherMina Ezzat Attya
Feb 22, 2021·Journal of Pediatric Urology·Süleyman Cüneyt KarakuşBurçin İrem Abas
May 31, 2021·Ecotoxicology and Environmental Safety·Peirong CaiJianchun Bian
Jun 25, 2021·Clinical Ophthalmology·Milena PahlitzschAnne Rübsam

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