Nest building is a novel method for indexing severity of alcohol withdrawal in mice

Behavioural Brain Research
G D GreenbergJohn C Crabbe

Abstract

Withdrawal after chronic ethanol (EtOH) affects body temperature, goal-directed behavior and motor function in mice and increases general central nervous system excitability. Nest-building tests have been used to assay these states but to this point have not been employed as measures of EtOH withdrawal severity. We first refined nest-scoring methods using a genetically heterogeneous stock of mice (HS/Npt). Mice were then made physically dependent following three days of chronic EtOH vapor inhalation to produce average blood EtOH concentrations (BECs) of 1.89 mg/mL. EtOH withdrawal affected the progression of nest building over time when mice were tested 2-4 days after removal from three days of chronic exposure to EtOH. In a separate group of mice, chronic EtOH vapor inhalation (BECs 1.84 mg/mL) suppressed nest building over days 1-2 but not days 2-3 of withdrawal. In a following experiment, EtOH withdrawal dose-dependently slowed recovery of nest building for up to 32 h. Finally, we determined that long-lasting nest-building deficits extend to mice undergoing withdrawal from a high dose (4 g/kg) of acute EtOH. Sex differences for nest building were absent following EtOH exposure. In mice naïve to EtOH treatments, male mice had...Continue Reading

References

Jun 1, 1994·Alcoholism, Clinical and Experimental Research·E S Terdal, J C Crabbe
Sep 13, 2001·The European Journal of Neuroscience·K GuentherJ N Rawlins
Oct 22, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sheila M FlemingMarie-Françoise Chesselet
Jul 12, 2005·Physiology & Behavior·Christopher L KliethermesJohn C Crabbe
Sep 3, 2005·Nature Reviews. Drug Discovery·John F Cryan, Andrew Holmes
Apr 5, 2007·Nature Protocols·Robert M J Deacon
Feb 12, 2008·Behavioural Brain Research·R M J DeaconJ N P Rawlins
Jun 20, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jennie R StevensonClyde W Hodge
Feb 13, 2010·Addiction Biology·Markus HeiligHoward C Becker
Sep 29, 2011·Psychopharmacology·Scott D PhilibinJohn C Crabbe
Feb 9, 2012·Alcoholism, Clinical and Experimental Research·John C CrabbePamela Metten
Jan 15, 2013·Physiology & Behavior·Brianna N GaskillJoseph P Garner
Feb 1, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Carine DournesGuy Griebel
Jan 17, 2014·Journal of Visualized Experiments : JoVE·Brianna N GaskillKathleen R Pritchett-Corning

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Citations

Mar 24, 2018·Addiction Biology·Zhe ShiHuanxing Su
Apr 25, 2018·Alcoholism, Clinical and Experimental Research·Harpreet SidhuCandice Contet
Dec 26, 2017·Frontiers in Aging Neuroscience·Wenwen LianGuanhua Du
Jul 25, 2019·Laboratory Animals·Kerstin SchwabeHeidrun Potschka

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