PMID: 9542943May 23, 1998Paper

Nested PCR assay for detection of granulocytic ehrlichiae

Journal of Clinical Microbiology
R F MassungJ G Olson

Abstract

A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis. Five of the seven suspected cases were positive by the PCR assay using DNA extracted from whole blood as the template, compared with a serologic assay that identified only one positive sample. The PCR assay using DNA extracted from the corresponding serum samples as the template identified three positive samples. The sensitivity of the assay on human samples was examined, and the limit of detection was shown to be fewer than 2 copies of the 16S rRNA gene. The application of the assay to nonhuman samples demonstrated products amplified from template DNA extracted from Ixodes scapularis ticks collected in Rhode Island and from EDTA-blood specimens obtained from white-tailed deer in Maryland. All PCR products were sequenced and identified as specific to granulocytic ehrlichiae. A putative variant granulocytic ehrlichia 16S rRNA gene sequence was detected among products amplified from both the ticks and the deer blood specimens.

References

Dec 1, 1991·Journal of Clinical Microbiology·J E DawsonC W Duntley
Dec 1, 1991·Journal of Clinical Microbiology·B E AndersonK H Wilson
Apr 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S J RodgersC A Baldwin
Apr 2, 1987·The New England Journal of Medicine·K MaedaJ E McDade
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Dec 2, 1995·Lancet·K J SumptionB A Dale
Oct 1, 1995·The Journal of Infectious Diseases·P PancholiD H Persing
May 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J S Dumler, J S Bakken
Sep 16, 1995·Lancet·P BrouquiD Raoult
Jul 1, 1994·Journal of Clinical Microbiology·Z IqbalY Rikihisa
May 1, 1994·Annals of Internal Medicine·E D EverettG McDonald
Mar 1, 1994·Journal of Clinical Microbiology·S M ChenD H Walker
Jan 25, 1996·The New England Journal of Medicine·J L GoodmanU G Munderloh
Oct 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C J HardaloJ S Dumler
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·S R TelfordD H Persing
Sep 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A S GewirtzD H Persing
Dec 1, 1996·Journal of Clinical Microbiology·W L Chang, M J Pan
Oct 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J S BakkenB E Kristiansen
Dec 1, 1996·Annals of Internal Medicine·M E Aguero-RosenfeldJ S Dumler
Apr 1, 1997·Journal of Clinical Microbiology·M T YehR F Massung
Jun 1, 1997·Journal of Clinical Microbiology·E A BelongiaJ J Kazmierczak
Jun 1, 1997·Journal of Clinical Microbiology·M PetrovecJ S Dumler

❮ Previous
Next ❯

Citations

May 28, 2002·Scandinavian Journal of Infectious Diseases·Anneli BjöersdorffIngvar Eliasson
May 9, 2002·Emerging Infectious Diseases·Robert F MassungThomas N Mather
May 23, 2003·The Journal of Parasitology·Christopher H GorhamLance A Durden
Jul 16, 2003·Annals of the New York Academy of Sciences·Joshua W Courtney, Robert F Massung
Oct 13, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B MantelliA Rizzoli
Nov 23, 2006·Annals of the New York Academy of Sciences·Lénaïg HalosMuriel Vayssier-Taussat
Feb 7, 2008·Emerging Infectious Diseases·Katia AbarcaTeresa Azócar
Nov 1, 2008·Vector Borne and Zoonotic Diseases·A S SantosJ S Dumler
Dec 22, 2009·Vector Borne and Zoonotic Diseases·Violaine CottéMuriel Vayssier-Taussat
Feb 18, 2011·Vector Borne and Zoonotic Diseases·Cornelia SilaghiSteffen Rehbein
Aug 17, 2011·Parasites & Vectors·Cornelia SilaghiKurt Pfister
Nov 10, 2011·FEMS Immunology and Medical Microbiology·Katja Strasek SmrdelTatjana Avsic Zupan
Mar 13, 2012·Applied and Environmental Microbiology·R De SousaM S Núncio
May 29, 2014·The American Journal of Tropical Medicine and Hygiene·Felicia KeesingRichard S Ostfeld
Apr 4, 2019·Parasite : Journal De La Société Française De Parasitologie·Toufic AklLionel Zenner
Dec 17, 2019·Vector Borne and Zoonotic Diseases·Turabi Gunes, Mehmet Ataş
Mar 26, 1999·The Journal of Small Animal Practice·A BjöersdorffR F Massung
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·Anneli BjöersdorffIngvar Eliasson
Jul 2, 2004·Annals of Tropical Medicine and Parasitology·T SréterD Kálmán
Oct 18, 2003·Current Opinion in Hematology·Ritchard G Cable, David A Leiby
Oct 24, 2008·Vector Borne and Zoonotic Diseases·Giovanna CarpiAnnapaola Rizzoli
Dec 2, 2005·Emerging Infectious Diseases·Robert F MassungRichard L Dryden
Aug 18, 2009·Expert Review of Anti-infective Therapy·Rachael J ThomasJason A Carlyon
Jul 24, 2008·Experimental & Applied Acarology·Janet FoleyMike B Teglas
May 3, 2013·Acta Veterinaria Scandinavica·Thomas HennigerFriederike D von Loewenich
May 3, 2012·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Kerry L Clark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.