PMID: 11933268Apr 5, 2002Paper

Nested PCR for diagnosis of tuberculous lymphadenitis and PCR-SSCP for identification of rifampicin resistance in fine-needle aspirates

Diagnostic Cytopathology
Gyungyub GongShin Kwang Khang

Abstract

An accurate diagnosis of tuberculosis and multidrug resistance is important for the control of tuberculosis, which remains a major public health problem. Fine-needle aspiration (FNA) has provided an alternative tool for bacterial examination. This study was performed to investigate the usefulness of one-step polymerase chain reaction (PCR) and PCR-SSCP as a routine test for the detection of Mycobacterium tuberculosis and rifampicin-resistant strain in FNA. Ziehl-Neelsen stain (Z-N) and PCR were processed using the aspirates of tuberculous lymphadenitis for the detection of M. tuberculosis. PCR-SSCP was done for the identification of rpoB mutation. M. tuberculosis was detected in 49/63 (77.8%) by PCR and 25/63 (39.7%) by Z-N. There were 26 cases with PCR(+)/Z-N(-) and two cases with PCR(-)/Z-N(+). Twelve cases showed negativity against both. In 7/22 (31.8%), rpoB mutation was observed. In conclusion, PCR is more sensitive in the detection of M. tuberculosis in FNA than Z-N. PCR-SSCP could also be used in FNA in the prediction of multidrug resistance.

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Citations

Jun 6, 2003·Best Practice & Research. Clinical Rheumatology·A N Malaviya, P P Kotwal
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Mar 1, 2012·Internal Medicine Journal·J KnoxH Karunajeewa
Jul 4, 2003·Scandinavian Journal of Infectious Diseases·Mohammed Ahmed YassinGetahun Abate
Aug 11, 2005·Journal of Medical Microbiology·Ruma PahwaBhudev C Das
Jul 27, 2007·Scandinavian Journal of Infectious Diseases·Mehrdad HasibiMehdi Abouzari

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