Nested polymerase chain reaction for detection of the avian leukosis virus causing so-called fowl glioma

Avian Pathology : Journal of the W.V.P.A
Hitoshi HataiTakashi Umemura

Abstract

The complete nucleotide sequence of the avian leukosis virus causing so-called fowl glioma has been previously determined. Primers were designed for detection of the fowl glioma-causal virus (FGV) based on the 3' untranslated region of the viral genome. The provirus and viral RNA of FGV were specifically detected in various organs and tissues, including feather pulp, from experimentally infected birds using nested polymerase chain reaction (PCR) and reverse transcription nested PCR. The prevalence of FGV was evaluated in 131 Japanese fowls of a zoological garden in Japan based on the detection of the FGV genome in feather pulp using PCR and the detection of viral antigen in faeces by enzyme-linked immunosorbent assay. FGV proviral DNA was detected in feather pulp of 52 birds (39.7%) by nested PCR. Later, nine dead birds from among the 52 were histologically diagnosed as having fowl glioma and found to have the proviral DNA in the affected brain. These results demonstrated that the PCR-based detection of FGV in feather pulp is useful for epidemiological studies on fowl glioma.

References

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Mar 9, 2005·Veterinary Pathology·T ToyodaT Umemura

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Citations

Jul 2, 2011·Journal of Neuro-oncology·Alexander KofmanRoger Abounader
Jul 20, 2010·Tropical Animal Health and Production·Mostafa RajabzadehAli Mohammadi
Apr 9, 2008·Avian Pathology : Journal of the W.V.P.A·Hitoshi HataiTakashi Umemura
Aug 23, 2011·Avian Pathology : Journal of the W.V.P.A·Sayuri NakamuraTakashi Umemura
Jun 19, 2012·Avian Pathology : Journal of the W.V.P.A·Akihiro OchiTakashi Umemura
Jun 6, 2008·The Journal of Veterinary Medical Science·Hitoshi HataiTakashi Umemura
Feb 7, 2014·Avian Pathology : Journal of the W.V.P.A·Sayuri NakamuraAsumi Abe
Jul 1, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hitoshi HataiTakashi Umemura
Aug 30, 2008·Veterinary Pathology·A OchiT Umemura
Apr 18, 2020·Avian Pathology : Journal of the W.V.P.A·Hayate NishiuraKenji Ochiai

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Datasets Mentioned

BETA
AB112960
Z46390
L08165

Methods Mentioned

BETA
PCR
light microscopy
electrophoresis
enzyme-linked immnosorbent assay
ELISA
electron microscopy

Software Mentioned

GENETYXMAC

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