PMID: 9531637Jun 6, 1998Paper

Net efflux of citrate in Penicillium simplicissimum is mediated by a transport protein

Archives of Microbiology
M GallmetzerWolfgang Burgstaller

Abstract

Penicillium simplicissimum excreted citrate, isocitrate, and succinate when grown in a strongly buffered medium [1 M Mes (pH 6) or 1 M Hepes (pH 7.3)]. Growth in a weakly buffered medium did not lead to citrate excretion despite a similar intracellular citrate concentration. When nongrowing, citrate-excreting hyphae were aerated in a glucose solution, the following steady-state intracellular concentrations of organic acids were measured: succinate (25 mM); citrate, isocitrate, malate, and fumarate (all less than 5 mM). After 2 h of incubation, the extracellular concentrations of these acids were [micromol (g dry wt.)-1]: isocitrate [100], citrate [60], succinate [30], and malate, fumarate, and alpha-ketoglutarate [<5]. The excretion of citrate was due neither to an unspecific change in the permeability of the plasma membrane nor to simple diffusion of undissociated citric acid. The involvement of a transport protein in citrate excretion was indicated because N-ethylmaleimide and sodium azide inhibited citrate excretion strongly despite an unchanged outward-directed citrate gradient. Arguments are given why efflux via a citrate uptake carrier is not considered probable. These results indicate that citrate is excreted by P. simpl...Continue Reading

Citations

Jul 20, 2002·FEMS Microbiology Letters·Martin SimkovicL'udovít Varecka
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·PR RyanDL Jones
Jun 5, 2002·FEMS Microbiology Letters·Martin GallmetzerWolfgang Burgstaller
Nov 5, 2019·Frontiers in Microbiology·Pamela VrablWolfgang Burgstaller

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