Netrin-1 Preserves Blood-Brain Barrier Integrity Through Deleted in Colorectal Cancer/Focal Adhesion Kinase/RhoA Signaling Pathway Following Subarachnoid Hemorrhage in Rats

Journal of the American Heart Association
Zongyi XieJohn H Zhang

Abstract

Netrin-1 (NTN-1) has been established to be a novel intrinsic regulator of blood-brain barrier (BBB) maintenance. This study was carried out to investigate the potential roles of exogenous NTN-1 in preserving BBB integrity after experimental subarachnoid hemorrhage (SAH) as well as the underlying mechanisms of its protective effects. A total of 309 male Sprague-Dawley rats were subjected to an endovascular perforation model of SAH. Recombinant NTN-1 was administered intravenously 1 hour after SAH induction. NTN-1 small interfering RNA or Deleted in Colorectal Cancer small interfering RNA was administered intracerebroventricular at 48 hours before SAH. Focal adhesion kinase inhibitor was administered by intraperitoneal injection at 1 hour prior to SAH. Neurological scores, brain water content, BBB permeability, RhoA activity, Western blot, and immunofluorescence staining were evaluated. The expression of endogenous NTN-1 and its receptor Deleted in Colorectal Cancer were increased after SAH. Administration of exogenous NTN-1 significantly reduced brain water content and BBB permeability and ameliorated neurological deficits at 24 and 72 hours after SAH. Exogenous NTN-1 treatment significantly promoted phosphorylated focal adhesi...Continue Reading

References

May 4, 2002·Stroke; a Journal of Cerebral Circulation·Jan ClaassenStephan A Mayer
Feb 22, 2003·Circulation Research·Dusko IlicCaroline H Damsky
Oct 21, 2004·Nature Neuroscience·Xiu-Rong RenWen-Cheng Xiong
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kye Won ParkDean Y Li
Dec 2, 2004·Nature Reviews. Cancer·Hirofumi Arakawa
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jul 1, 2006·Science·Brent D WilsonDean Y Li
Jul 20, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yi YangGary A Rosenberg
Mar 16, 2007·Nature Reviews. Molecular Cell Biology·Vincenzo Cirulli, Mayra Yebra
Sep 18, 2007·Journal of Neuroscience Methods·Takashi SugawaraJohn H Zhang
May 8, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yongfeng FanGuo-Yuan Yang
Jan 12, 2010·Thrombosis and Haemostasis·Cora M L BeckersGeerten P van Nieuw Amerongen
Mar 20, 2010·Cardiovascular Research·Volker SpindlerJens Waschke
Jul 24, 2010·Stroke; a Journal of Cerebral Circulation·Claire AllenUlvi Bayraktutan
May 10, 2011·Microvascular Research·Katie L Grinnell, Elizabeth O Harrington
May 12, 2011·Development·Karen Lai Wing SunTimothy E Kennedy
Jun 19, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tracy Thennes SchmidtDolly Mehta
Aug 22, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Xiaosong HeGuo-Yuan Yang
Sep 7, 2013·Stroke; a Journal of Cerebral Circulation·Basak Caner TopkoruJohn H Zhang
Oct 1, 2013·Progress in Neurobiology·Sheng ChenJohn H Zhang
Nov 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer S GoldmanTimothy E Kennedy
Jan 1, 2015·Stroke; a Journal of Cerebral Circulation·Yujie ChenJohn H Zhang
Apr 24, 2015·Brain : a Journal of Neurology·Cornelia PodjaskiAlexandre Prat
Jul 26, 2015·Cardiovascular Research·Kerry LayneGabriella Passacquale

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Citations

Mar 17, 2018·Molecular Neurobiology·J J BrudvigJ M Weimer
Apr 15, 2019·Journal of Neuroinflammation·Diana G BohannonWoong-Ki Kim
Jan 22, 2020·Neural Regeneration Research·Da-Qiang KeHui Liang
Jul 10, 2018·Molecular Neurobiology·T Peeyush KumarSpiros L Blackburn
Mar 11, 2021·Cellular and Molecular Life Sciences : CMLS·Timothy J A Chico, Elisabeth C Kugler
Mar 21, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yun XieDezhi Bo

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Methods Mentioned

BETA
transfection
fluorescence microscopy
electrophoresis
Assay

Software Mentioned

ImageJ
LASX
GraphPad Prism
SigmaPlot

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