Netrin-1/DCC Signaling Differentially Regulates the Migration of Pax7, Nkx6.1, Irx2, Otp, and Otx2 Cell Populations in the Developing Interpeduncular Nucleus

Frontiers in Cell and Developmental Biology
Isabel M García-GuillénPilar Aroca

Abstract

The interpeduncular nucleus (IPN) is a hindbrain structure formed by three main subdivisions, the prodromal (Pro) domain located at the isthmus (Ist), and the rostral and caudal interpeduncular domains (IPR, IPC) within rhombomere 1 (r1). Various cell populations can be detected in the IPN through the expression of the Nkx6.1, Otp, Otx2, Pax7, and/or Irx2 transcription factors. These cell populations follow independent dorsoventral tangential and radial migratory routes targeting the ventral paramedian region of Ist and r1. Here we set out to examine the influence of the Netrin-1/DCC pathway on these migrations, since it is known to regulate other processes of neuronal migration in the brain. To this end, we analyzed IPN development in late gestational wild-type and DCC-/- mice, using mainly in situ hybridization (ISH) to identify the cells expressing each of the aforementioned genes. We found that the migration of Nkx6.1+ and Irx2+ cells into the Pro domain was strongly disrupted by the loss of DCC, as occurred with the migration of Pax7+, Irx2+, and Otp+ cells that would normally form the IPR. In addition, there was mild impairment of the migration of the Pax7+ and Otx2+ cells that form the IPC. These results demonstrate that...Continue Reading

References

Sep 1, 1979·The Journal of Comparative Neurology·M Herkenham, W J Nauta
Jul 1, 1986·The Journal of Comparative Neurology·H J GroenewegenW J Nauta
May 15, 1994·Genes & Development·L HedrickB Vogelstein
Mar 7, 1996·Nature·P H CrossleyG R Martin
Jan 1, 1996·Methods in Cell Biology·M A NietoD G Wilkinson
Oct 18, 1996·Cell·K Keino-MasuM Tessier-Lavigne
Jun 2, 2001·The EMBO Journal·F LlambiP Mehlen
Oct 10, 2003·Development·Myriam MüllerMaike Sander
May 7, 2004·The European Journal of Neuroscience·Lucas LecourtierPeter H Kelly
Mar 2, 2005·Nature Reviews. Neuroscience·Marysia Placzek, James Briscoe
Aug 23, 2005·Brain Research. Brain Research Reviews·Pilar Aroca, Luis Puelles
Aug 23, 2005·Brain Research. Brain Research Reviews·Evelyne Bloch-GallegoMatías Hidalgo-Sánchez
Sep 6, 2008·Molecular and Cellular Neurosciences·Ming ShiYu-Qiang Ding
May 5, 2009·Molecular and Cellular Neurosciences·Séverine MarcosEvelyne Bloch-Gallego
May 12, 2009·Current Opinion in Neurobiology·Alain Chédotal, Filippo M Rijli
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Oscar MarínLi-Huei Tsai
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Greg J Bashaw, Rüdiger Klein
Jun 19, 2010·Nature Reviews. Neuroscience·Okihide Hikosaka
Apr 13, 2011·Journal of Psychiatry & Neuroscience : JPN·Cecilia Flores
Mar 16, 2013·Brain Structure & Function·J A Moreno-BravoE Puelles
Aug 14, 2013·Annual Review of Cell and Developmental Biology·Irina EvsyukovaE S Anton
May 28, 2014·Neuroscience Letters·Alanna GrantCecilia Flores
Apr 18, 2015·Progress in Biophysics and Molecular Biology·L FinciJ-H Wang
May 17, 2017·The Journal of Comparative Neurology·Charles WatsonLuis Puelles
Jun 2, 2017·Frontiers in Neural Circuits·Claudius F KratochwilFilippo M Rijli
Feb 15, 2018·Cell Reports·Andrea R YungLisa V Goodrich
Dec 30, 2018·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nuria Ruiz-ReigFabien D'Autréaux

❮ Previous
Next ❯

Citations

Jun 8, 2021·Frontiers in Cell and Developmental Biology·Lara López-GonzálezLuis Puelles

❮ Previous
Next ❯

Datasets Mentioned

BETA
AK133693
BC029750

Methods Mentioned

BETA
Sections
genotyping
transgenic

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.