Netropsin, a minor groove binding ligand: a potential radioprotective agent

Radiation Research
K MishraN K Chaudhury

Abstract

Minimizing radiation-induced damages in DNA is an important aspect in the development of chemical radioprotectors. The aim of this study was to evaluate the possible radioprotective ability of the DNA minor groove binding ligand netropsin in an aqueous solution of plasmid DNA (pBR322) and to compare its efficacy with that of Hoechst 33258, a known radioprotector. The radiochemical parameters D(0), G(SSB) and DMF were calculated in pBR322 DNA. Based on a comparison of the DMFs of netropsin and Hoechst 33258, netropsin appeared to be the better radioprotector. The ligand binding site accessibility of the restriction enzyme EcoRI at the ligand-pBR322 complex was assessed using a restriction-digestion assay in irradiated solutions. A distinct ligand-bound site protection in netropsin-DNA was observed in irradiated solutions. However, no site protection was observed in the presence of Hoechst 33258. The possible role of ligand-induced structural stabilization in irradiated aqueous solutions was also investigated using netropsin-calf thymus DNA melting temperature measurements. The greater radioprotective ability of netropsin in solutions of DNA was suggested to be due to its higher binding affinity and its ability to provide higher ...Continue Reading

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Citations

Jul 6, 2013·Mutation Research·Amit AlokN K Chaudhury
Sep 9, 2011·Radiation Research·K MishraN K Chaudhury
Feb 12, 2015·Nucleic Acids Research·Kiran ChintakayalaDavid C Grainger
Nov 5, 2020·Biomedicines·Elena ObradorAlegría Montoro
Sep 16, 2020·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Amit Alok, Paban K Agrawala
Nov 11, 2020·Chemico-biological Interactions·Deepti SharmaHimanshu Ojha
Jun 29, 2021·Free Radical Research·Rahul CheckerSantosh K Sandur
Jul 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Krishna N MishraGhazi A Alsbeih

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Methods Mentioned

BETA
electrophoresis
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